Anti-Phospholipase A2 抗体 (ab73406)

製品の概要

  • 製品名Anti-Phospholipase A2 antibody
    Phospholipase A2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Phospholipase A2
  • アプリケーション適用あり: IHC-P, WB, ICC/IFmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Cow, Dog, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 700 to the C-terminus of Human Phospholipase A2.

    (Peptide available as ab86259.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in HeLa Whole Cell Lysate - Bleomycin Treated (40U/ml)

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab73406 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 85 kDa).
ICC/IF Use a concentration of 1 µg/ml.

ターゲット情報

Anti-Phospholipase A2 antibody 画像

  • Anti-Phospholipase A2 antibody (ab73406) at 1 µg/ml + HeLa Whole Cell Lysate - Bleomycin Treated (40U/ml) at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 85 kDa
    Observed band size : 100 kDa (why is the actual band size different from the predicted?)
  • ICC/IF image of ab73406 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab73406, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) Hek293, HepG2 and MCF7 cells at 1µg/ml, and in 100% methanol fixed (5 min) HeLa cells at 1µg/ml.
  • IHC image of Phospholipsae A2 staining in humam colon carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab73406, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-Phospholipase A2 antibody (ab73406) 使用論文

ab73406 has not yet been referenced specifically in any publications.

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Das Labor, das Anti-Phospholipase A2Antikoerper ab23705 herstellt, hat bestätigt, dass das Immunogen das Volllängenprotein sPLA2 (human Type IIA) mit der SwissProtID P14555.2 ist. Damit ist dieser A...

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DISCOUNT CODE: *** Expiration date: May 3rd, 2012 I am very pleased to hear you would like to accept our offer and test ab73406 in mouse. This code will give you 1 freeprimary antibodybefore the expiration date. To redeem this offer, please submit an A...

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Thank you for contacting us. We do not currently carry an antibody that meets your exact specifications, however you may be interested in ab73406. This antibody is raised in rabbit, and predicted to react with mouse. The immunogen sequence does not ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"