The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration. PubMed: 20584290
1/500 - 1/1000. Detects a band of approximately 110 kDa (predicted molecular weight: 85 kDa).
Use at an assay dependent concentration.
Selectively hydrolyzes arachidonyl phospholipids in the sn-2 position releasing arachidonic acid. Together with its lysophospholipid activity, it is implicated in the initiation of the inflammatory response.
Expressed in various tissues such as macrophages, platelets, neutrophils, fibroblasts and lung endothelium.
Contains 1 C2 domain. Contains 1 PLA2c domain.
The N-terminal C2 domain associates with lipid membranes upon calcium binding. It modulates enzyme activity by presenting the active site to its substrate in response to elevations of cytosolic Ca(2+).
Activated by phosphorylation at both Ser-505 and Ser-727.
Cytoplasm. Cytoplasmic vesicle. Translocates to membrane vesicles in a calcium-dependent fashion.
ab58375 at 1/50-1/100 dilution staining Phospholipase A2 in human colon carcinoma by Immunohistochemistry, Paraffin embedded tissue, in the absence or presence of the immunising peptide.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Phospholipase A2 antibody (ab58375)This image is courtesy of an anonymous Abreview
Immunohistochemical analysis of Human spleen tissue, staining Phospholipase A2 with ab58375.
Tissue was fixed with formaldehyde and blocked with blocking solution for 10 minutes at 37°C; antigen retrieval was by heat mediation in a cell conditioner. Samples were incubated with primary antibody (undiluted) for 1 hour at 37°C. A biotinylated-conjugated goat anti-rabbit polyclonal IgG was used as the secondary antibody.