Anti-Phospholamban (phospho S16) 抗体 (ab15000)

製品の概要

  • 製品名
    Anti-Phospholamban (phospho S16) antibody
    Phospholamban 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Phospholamban (phospho S16)
  • 特異性
    Multiple bands can be observed in addition to the 25kD pentamer for PLB. Monomeric, dimeric, trimeric forms, etc. can be observed if the preparations are boiled before SDS-PAGE. This antibody also can display non-specific bands at 75 and 100kD in some lysate preparations. Note: Do not boil cardiac preparation. Boiling may reduce the 25kD pentamer in to tri-, di- or monomers. In addition, phosphorylation may disrupt the pentamer into dimers and monomers.
  • アプリケーション
    適用あり: ICC/IF, IHC-P, WB, IHC-Frmore details
  • 種交差性
    交差種: Mouse, Rat, Rabbit, Hamster, Cow, Human, Pig, Chinese hamster
    交差が予測される動物種: Dog
  • 免疫原

    KLH-conjugated, synthetic peptide corresponding to amino acids 14-25 of human cardiac phospholamban (

    RA[pS]TIEMPQQAR-C

    ) with an added C-terminal cysteine. The immunizing sequence is identical in mouse, rat, rabbit, pig and dog.

  • ポジティブ・コントロール
    • Bovine cardiac preparation.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab15000 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
WB Use a concentration of 0.5 - 2 µg/ml. Predicted molecular weight: 6.24 kDa.
IHC-Fr Use at an assay dependent concentration.

ターゲット情報

  • 機能
    Reversibly inhibits the activity of ATP2A2 in cardiac sarcoplasmic reticulum by decreasing the apparent affinity of the ATPase for Ca(2+). Modulates the contractility of the heart muscle in response to physiological stimuli via its effects on ATP2A2. Modulates calcium re-uptake during muscle relaxation and plays an important role in calcium homeostasis in the heart muscle. The degree of ATP2A2 inhibition depends on the oligomeric state of PLN. ATP2A2 inhibition is alleviated by PLN phosphorylation.
  • 組織特異性
    Heart muscle (at protein level).
  • 関連疾患
    Cardiomyopathy, dilated 1P
    Cardiomyopathy, familial hypertrophic 18
  • 配列類似性
    Belongs to the phospholamban family.
  • 翻訳後修飾
    Phosphorylation by PKA abolishes the inhibition of ATP2A2-mediated calcium uptake. Phosphorylated at Thr-17 by CaMK2, and in response to beta-adrenergic stimulation. Phosphorylation by DMPK may stimulate sarcoplasmic reticulum calcium uptake in cardiomyocytes.
  • 細胞内局在
    Endoplasmic reticulum membrane. Sarcoplasmic reticulum membrane. Mitochondrion membrane. Membrane. Colocalizes with HAX1 at the endoplasmic reticulum (PubMed:17241641). Colocalizes with DMPK a the sarcoplasmic reticulum (PubMed:15598648).
  • Information by UniProt
  • 参照データベース
  • 別名
    • Cardiac phospholamban antibody
    • CMD1P antibody
    • CMH18 antibody
    • PLB antibody
    • Pln antibody
    • PPLA_HUMAN antibody
    see all

画像

  • All lanes : Anti-Phospholamban (phospho S16) antibody (ab15000) at 0.5 µg/ml

    Lane 1 : Bovine cardiac preparation treated with PKA
    Lane 2 : Untreated bovine cardiac preparation


    Predicted band size : 6.24 kDa
    Additional bands at : 14 kDa (possible multimer),23 kDa (possible multimer).
  • ab15000 staining Phospholamban (phospho S16) in Rat heart tissue by Immunohistochemistry (Frozen sections). The sections were fixed in Acetone prior to blocking with neat SuperBlock Blocking Buffer for 20 minutes at 23°C. The primary antibody was diluted 1/50 in PBS and incubated with the sample for 12 hours at 4°C. ab6720 - Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Biotin) was used as the secondary antibody, diluted 1/100.

    See Abreview

参考文献

This product has been referenced in:
  • Wu Y  et al. Cardiac Protection of Valsartan on Juvenile Rats with Heart Failure by Inhibiting Activity of CaMKII via Attenuating Phosphorylation. Biomed Res Int 2017:4150158 (2017). Read more (PubMed: 28536695) »
  • Ahmad F  et al. Regulation of sarcoplasmic reticulum Ca2+ ATPase 2 (SERCA2) activity by phosphodiesterase 3A (PDE3A) in human myocardium: phosphorylation-dependent interaction of PDE3A1 with SERCA2. J Biol Chem 290:6763-76 (2015). Read more (PubMed: 25593322) »

See all 16 Publications for this product

レビューと Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Sample
Mouse Cell (myocyte)
Specification
myocyte
Permeabilization
No
Fixative
Acetone
Username

Abcam user community

Verified customer

投稿 Jun 17 2013

Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (15)
Sample
Human Tissue lysate - whole (Heart)
Specification
Heart
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Verified customer

投稿 Jun 10 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Pig Tissue lysate - whole (Heart)
Loading amount
7.5 µg
Specification
Heart
Gel Running Conditions
Reduced Denaturing (14% PAGE)
Blocking step
membrane blocking solution (Invitrogen) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C
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投稿 Dec 19 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (heart)
Specification
heart
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: universal decloaker
Permeabilization
No
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
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投稿 Nov 27 2012

Thank you for contacting us. We have not tested these antibodies in guinea pig and therefore cannot guarantee they will work, however there is high homology between the immunogen and the guinea pig sequence for all three. Normally we predictlikely reac...

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Thank you for contacting Abcam.

I would be very happy to help answer your questions:

1 - What is the difference between ab86930 and ab85146? Which is more suitable for guinea pig?

- Both antibodies target different areas of t...

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I am sorry to hear that you have been experiencing problems using this product in the application that you wish. Unfortunately, the attachment has not come through successfully. Could you please resend it to me? It is much appreciated.

In ord...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (heart)
Specification
heart
Fixative
Acetone
Permeabilization
No
Blocking step
SuperBlock Blocking Buffer as blocking agent for 20 minute(s) · Concentration: 100% · Temperature: 23°C
Username

Abcam user community

Verified customer

投稿 Jan 19 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Tissue lysate - whole (heart tissue)
Loading amount
20 µg
Specification
heart tissue
Gel Running Conditions
Reduced Denaturing (15%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

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Verified customer

投稿 Nov 04 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Chinese Hamster Cell lysate - whole cell (CHO cell line)
Loading amount
20 µg
Specification
CHO cell line
Gel Running Conditions
Reduced Denaturing (15% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

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Verified customer

投稿 Jun 17 2009

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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