Anti-Phospholamban 抗体 (ab86930)
Key features and details
- Rabbit polyclonal to Phospholamban
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Phospholamban antibody
Phospholamban 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Phospholamban -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide corresponding to Human Phospholamban aa 2-16 (N terminal).
Sequence:EKVQYLTRSAIRRAS
Database link: P26678 -
ポジティブ・コントロール
- WB: Rat heart tissue lysate. CEM, MCF-7, HT1080, and HeLa cell lysates
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
バッファー
Preservatives: 0.025% Sodium azide, 0.025% Thimerosal (merthiolate)
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab86930の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 6 kDa.
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特記事項 |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 6 kDa. |
ターゲット情報
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機能
Reversibly inhibits the activity of ATP2A2 in cardiac sarcoplasmic reticulum by decreasing the apparent affinity of the ATPase for Ca(2+). Modulates the contractility of the heart muscle in response to physiological stimuli via its effects on ATP2A2. Modulates calcium re-uptake during muscle relaxation and plays an important role in calcium homeostasis in the heart muscle. The degree of ATP2A2 inhibition depends on the oligomeric state of PLN. ATP2A2 inhibition is alleviated by PLN phosphorylation. -
組織特異性
Heart muscle (at protein level). -
関連疾患
Cardiomyopathy, dilated 1P
Cardiomyopathy, familial hypertrophic 18 -
配列類似性
Belongs to the phospholamban family. -
翻訳後修飾
Phosphorylation by PKA abolishes the inhibition of ATP2A2-mediated calcium uptake. Phosphorylated at Thr-17 by CaMK2, and in response to beta-adrenergic stimulation. Phosphorylation by DMPK may stimulate sarcoplasmic reticulum calcium uptake in cardiomyocytes. -
細胞内局在
Endoplasmic reticulum membrane. Sarcoplasmic reticulum membrane. Mitochondrion membrane. Membrane. Colocalizes with HAX1 at the endoplasmic reticulum (PubMed:17241641). Colocalizes with DMPK a the sarcoplasmic reticulum (PubMed:15598648). - Information by UniProt
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参照データベース
- Entrez Gene: 5350 Human
- Entrez Gene: 18821 Mouse
- Entrez Gene: 64672 Rat
- Omim: 172405 Human
- SwissProt: P26678 Human
- SwissProt: P61014 Mouse
- SwissProt: P61016 Rat
- Unigene: 170839 Human
see all -
別名
- Cardiac phospholamban antibody
- CMD1P antibody
- CMH18 antibody
see all
画像
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All lanes : Anti-Phospholamban antibody (ab86930) at 0.5 µg/ml
Lanes 1-2 : Rat Heart Tissue Lysate at 50 µg
Lane 3 : CEM Whole Cell Lysate at 40 µg
Lane 4 : MCF-7 Whole Cell Lysate at 40 µg
Lane 5 : HT1080 Whole Cell Lysate at 40 µg
Lane 6 : HeLa Whole Cell Lysate at 40 µg
Predicted band size: 6 kDa -
All lanes : Anti-Phospholamban antibody (ab86930) at 1 µg/ml
All lanes : Rat heart tissue lysate
Predicted band size: 6 kDa
Observed band size: 11 kDa why is the actual band size different from the predicted?
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (7)
ab86930 は 7 報の論文で使用されています。
- Miranda-Silva D et al. Disturbed cardiac mitochondrial and cytosolic calcium handling in a metabolic risk-related rat model of heart failure with preserved ejection fraction. Acta Physiol (Oxf) 228:e13378 (2020). PubMed: 31520455
- Silveira AC et al. Exercise Training Restores Cardiac MicroRNA-1 and MicroRNA-29c to Nonpathological Levels in Obese Rats. Oxid Med Cell Longev 2017:1549014 (2017). PubMed: 29138674
- Yavari A et al. Mammalian ?2 AMPK regulates intrinsic heart rate. Nat Commun 8:1258 (2017). PubMed: 29097735
- Seldin MM et al. A systems genetics approach identifies Trp53inp2 as a link between cardiomyocyte glucose utilization and hypertrophic response. Am J Physiol Heart Circ Physiol 312:H728-H741 (2017). PubMed: 28235788
- Najafi A et al. Selective phosphorylation of PKA targets after ß-adrenergic receptor stimulation impairs myofilament function in Mybpc3-targeted HCM mouse model. Cardiovasc Res 110:200-14 (2016). PubMed: 26825555
- Melo SF et al. Exercise training restores the cardiac microRNA-1 and -214 levels regulating Ca2+ handling after myocardial infarction. BMC Cardiovasc Disord 15:166 (2015). WB ; Rat . PubMed: 26646371
- Locatelli J et al. Swimming training promotes cardiac remodeling and alters the expression of mRNA and protein levels involved in calcium handling in hypertensive rats. Life Sci 117:67-74 (2014). PubMed: 25283082