製品の概要

  • 製品名Anti-Phospho - (Ser/Thr) antibody
    Phospho - (Ser/Thr) 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Phospho - (Ser/Thr)
  • アプリケーション適用あり: ICC/IF, ELISA, IP, WBmore details
  • 種交差性
    交差種: Species independent
  • 免疫原

    Synthetic peptide corresponding to Phospho - (Ser/Thr) Phe . Immunogen = Synthetic phospho-serine/threonine-phenylalanine containing peptide.

  • ポジティブ・コントロール
    • Calyculin A treated A431 cells
  • 特記事項

    The immunogen used is specific phosphorylated serine or threonine amino acids surrounded by phenylalanine.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab17464 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/100.
ELISA 1/1000.
IP 1/100.
WB 1/1000.

Block with 5% nonfat milk, 0.1% Tween-20. Dilute primary antibody in 5% BSA, 0.1% Tween-20.

ターゲット情報

  • 関連性A hallmark of signal transduction pathways is the reversible phosphorylation of serine and threonine residues within specific sequences, or motifs, in target proteins. Specific signaling motifs include not only sequences that are recognized by protein kinases, but also those that are recognized by phosphorylation-dependent binding proteins like 14-3-3. These modular phosphoprotein interacting domains are critical elements in modulating, directing and amplifying intracellular communications. Many critical protein kinases can be regulated by phosphorylation at a specific serine or threonine surrounded by phenylalanine or tyrosine. For example, Akt, an important kinase that regulates cell survival, is activated by phosphorylation at Ser473, a site surrounded by phenylalanine and tyrosine. RSK1, p70 S6 K, and certain PKC isoforms also contain a similar consensus phosphorylation site. Phosphorylation of these sites is required for kinase activity.
  • 別名
    • Phosphoserine antibody
    • Phosphothreonine antibody

Anti-Phospho - (Ser/Thr) antibody 画像

  • Image from Gao X et al, PLoS Pathog 5:e1000708 (2009), Fig S3.

    To determine if NleH could phosphorylate RPS3, we conducted in vitro kinase assays with NleH and RPR3 and analyzed the results by Immunoblotting with ab17464, phospho-Ser/Thr-specific antibody, following separation by SDS-PAGE.
  • All lanes : Anti-Phospho - (Ser/Thr) antibody (ab17464) at 1/1000 dilution

    Lane 1 : Human THP-1 Whole Cell Extract
    Lane 2 : Mouse Total Lung Extract

    Lysates/proteins at 10 µg per lane.

    Secondary
    Alkaline phosphatase conjugated goat anti-rabbit antibody
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 5 minutes

    This image is courtesy of an anonymous Abreview

    See Abreview

  • All lanes : Anti-Phospho - (Ser/Thr) antibody (ab17464) at 1/1000 dilution

    Lane 1 : calyculin A treated A431 cells
    Lane 2 : calyculin A treated A431 cells

Anti-Phospho - (Ser/Thr) antibody (ab17464) 使用論文

This product has been referenced in:
  • Xu M  et al. ErbB2 and p38? MAPK mediate alcohol-induced increase in breast cancer stem cells and metastasis. Mol Cancer 15:52 (2016). Read more (PubMed: 27416801) »
  • Li HB  et al. Mitochondrial Ribosomal Protein L10 Associates with Cyclin B1/Cdk1 Activity and Mitochondrial Function. DNA Cell Biol 35:680-690 (2016). Human . Read more (PubMed: 27726420) »

See all 12 Publications for this product

Product Wall

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing
Sample Mouse Cell lysate - whole cell (hepatocytes)
Specification hepatocytes
Treatment 20 ug/ml poly(I:C)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

投稿 Jun 26 2014


The concentration of ab17464 Anti-Phosphoserine/threonine antibody lot GR112407 is 0.075mg

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLa)
Loading amount 100 µg
Specification HeLa
Treatment UV irradiation
Gel Running Conditions Reduced Denaturing (10%)
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

投稿 May 13 2013

Thank you for contacting us.

The ab9337 recognizes proteins phosphorylated on threonine residues. It does not cross-react with phosphotyrosine.

For ab15556, this antibody recognizes serine, threonine, and tyrosine phosphorylated pr...

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The antibody ab17464 has been qc tested for its specificity and we have found no problem with it. It is highly likely that the expressed protein you are using is not compatible with the antibodies, therefore I wo...

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It may well be that this antibody in vial might have degraded or might have lost the specificity due to unknown reasons. In order to avoid any further delays in your project I am happy to offer you eith...

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