Phosphate Assay Kit (Fluorometric) (ab102508)
Key features and details
- Assay type: Quantitative
- Detection method: Fluorescent
- Platform: Microplate reader
- Assay time: 2 hr
- Sample type: Cell Lysate, Other biological fluids, Plasma, Serum, Tissue Extracts, Urine
- Sensitivity: 0.1 nmol/well
製品の概要
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製品名
Phosphate Assay Kit (Fluorometric)
Phosphate キット 製品一覧 -
検出方法
Fluorescent -
サンプルの種類
Urine, Serum, Plasma, Other biological fluids, Tissue Extracts, Cell Lysate -
アッセイタイプ
Quantitative -
検出感度
> 0.1 nmol/well -
検出範囲
2 µM - 10 µM -
全工程の試験時間
2h 00m -
製品の概要
Abcam's Phosphate Assay Kit (Fluorometric) provides a sensitive, easy, quick means of assessing phosphate over a wide range of concentrations. In the assay, inorganic phosphate reacted with maltose to produce glucose in the present of a special enzyme. The glucose is specifically oxidized to generate a product that reacts with the OxiRed probe to generate fluorescence (Ex/Em=535/587nm). The kit can be used to detect Pi produced through reactions involving ATPases, GTPases, 5'-nucleotidase, protein phosphatases, acid and alkaline phosphatases, and phosphorylase, etc. from a variety of samples. Glucose background can be eliminated by doing a glucose control.
Visit our FAQs page for tips and troubleshooting.
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特記事項
This product is manufactured by BioVision, an Abcam company and was previously called K420 Phosphate Assay Kit (Fluorometric). K420-100 is the same size as the 100 test size of ab102508.
Inorganic phosphate (Pi) is one of the most important ions in biological systems. It functions in a variety of roles. One of the most important roles is as a molecular switch, turning enzyme activity on and off through the mediation of the various protein kinases and phosphatases in biological systems. A highly sensitive assay is desired to monitor Pi in variety samples or monitor Pi changes during kinase and phosphatase reactions.
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試験プラットフォーム
Microplate reader
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 100 tests Converter 1 vial Developer 1 vial Phosphate Assay Buffer 1 x 25ml Phosphate Standard 1 x 50µl Phosphate Substrate 1 x 0.2ml PicoProbe (in DMSO) 1 x 200µl -
研究分野
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関連性
Phosphate is one of the most important of the inorganic ions in biological systems. It functions in a variety of roles. One of the most important roles is as a molecular switch, turning enzyme activity on and off through the mediation of the various protein kinases and phosphatases in biological systems. Phosphate is also of great importance in mineralization processes and is a primary stimulus of algal blooms frequently found in bodies of fresh water, due to run-off from areas of high fertilizer use.
データシートおよび資料
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Datasheet download
参考文献 (3)
ab102508 は 3 報の論文で使用されています。
- Jackson EK et al. Alkaline Phosphatase Inhibitors Attenuate Renovascular Responses to Norepinephrine. Hypertension 69:484-493 (2017). PubMed: 28137984
- Erkizan HV et al. RNA helicase A activity is inhibited by oncogenic transcription factor EWS-FLI1. Nucleic Acids Res 43:1069-80 (2015). PubMed: 25564528
- Dai H et al. Transient binding of an activator BH3 domain to the Bak BH3-binding groove initiates Bak oligomerization. J Cell Biol 194:39-48 (2011). PubMed: 21727192