Anti-PHLPP2 抗体 (ab71973)
Key features and details
- Rabbit polyclonal to PHLPP2
- Suitable for: ICC/IF, WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-PHLPP2 antibody
PHLPP2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PHLPP2 -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WB, IPmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rabbit, Guinea pig, Rhesus monkey, Gorilla -
免疫原
Synthetic peptide corresponding to a region between residues 1275 and the C-terminus (residue 1323) of human PHLPP2 (NP_055835.2)
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab71973の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
Use at an assay dependent concentration.
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WB |
1/2000 - 1/10000. Detects a band of approximately 190 kDa (predicted molecular weight: 147 kDa).
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IP |
Use at 1-4 µg/mg of lysate.
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特記事項 |
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ICC/IF
Use at an assay dependent concentration. |
WB
1/2000 - 1/10000. Detects a band of approximately 190 kDa (predicted molecular weight: 147 kDa). |
IP
Use at 1-4 µg/mg of lysate. |
ターゲット情報
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機能
Protein phosphatase that mediates dephosphorylation of 'Ser-473' of AKT1, 'Ser-660' of PRKCB isoform beta-II and 'Ser-657' of PRKCA. AKT1 regulates the balance between cell survival and apoptosis through a cascade that primarily alters the function of transcription factors that regulate pro- and antiapoptotic genes. Dephosphorylation of 'Ser-473' of AKT1 triggers apoptosis and decreases cell proliferation. Also controls the phosphorylation of AKT3. Dephosphorylation of PRKCA and PRKCB leads to their destabilization and degradation. Inhibits cancer cell proliferation and may act as a tumor suppressor. -
組織特異性
In colorectal cancer tissue, expression is highest in the surface epithelium of normal colonic mucosa adjacent to the cancer tissue but is largely excluded from the crypt bases. Expression is lost or significantly decreased in 80% of tested tumors (at protein level). -
配列類似性
Contains 22 LRR (leucine-rich) repeats.
Contains 1 PH domain.
Contains 1 PP2C-like domain. -
細胞内局在
Cytoplasm. Membrane. Nucleus. In colorectal cancer tissue, expression is concentrated in the cytoplasm and nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 23035 Human
- Entrez Gene: 244650 Mouse
- Omim: 611066 Human
- SwissProt: Q6ZVD8 Human
- SwissProt: Q8BXA7 Mouse
- Unigene: 709458 Human
- Unigene: 23494 Mouse
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別名
- KIAA0931 antibody
- PH domain and leucine rich repeat protein phosphatase 2 antibody
- PH domain leucine rich repeat protein phosphatase like antibody
see all
画像
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All lanes : Anti-PHLPP2 antibody (ab71973) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Predicted band size: 147 kDa
Observed band size: 190 kDa why is the actual band size different from the predicted? -
Detection of PHLPP2 by Western Blot of Immunoprecipitate.
ab71973 at 1µg/ml staining PHLPP2 in HeLa whole cell lysate immunoprecipitated using ab71973 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: chemiluminescence with exposure time of 30 seconds.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (18)
ab71973 は 18 報の論文で使用されています。
- Shi X et al. Circular RNA ANAPC7 Inhibits Tumor Growth and Muscle Wasting via PHLPP2-AKT-TGF-β Signaling Axis in Pancreatic Cancer. Gastroenterology 162:2004-2017.e2 (2022). PubMed: 35176309
- Tantai J et al. TRIM46 activates AKT/HK2 signaling by modifying PHLPP2 ubiquitylation to promote glycolysis and chemoresistance of lung cancer cells. Cell Death Dis 13:285 (2022). PubMed: 35354796
- Huang J et al. ZDHHC22-mediated mTOR palmitoylation restrains breast cancer growth and endocrine therapy resistance. Int J Biol Sci 18:2833-2850 (2022). PubMed: 35541896
- Nayak RC et al. Nuclear Vav3 is required for polycomb repression complex-1 activity in B-cell lymphoblastic leukemogenesis. Nat Commun 13:3056 (2022). PubMed: 35650206
- Li Y et al. Novel mechanisms underlying inhibition of inflammation-induced angiogenesis by dexamethasone and gentamicin via PI3K/AKT/NF-κB/VEGF pathways in acute radiation proctitis. Sci Rep 12:14116 (2022). PubMed: 35982137