製品の概要

  • 製品名
  • 製品の詳細
    Goat polyclonal to PHLPP2
  • アプリケーション
    適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Cynomolgus monkey
  • 免疫原

    Synthetic peptide:

    C-PHEEDRTEPPEEFD

    , corresponding to C terminal amino acids 1307-1320 of Human PHLPP2

  • ポジティブ・コントロール
    • Human Brain (Amygdala, Hippocampus, Substantia Nigra) lysate

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab62830 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 147 kDa (predicted molecular weight: 147 kDa).
ELISA 1/32000.

ターゲット情報

  • 機能
    Protein phosphatase that mediates dephosphorylation of 'Ser-473' of AKT1, 'Ser-660' of PRKCB isoform beta-II and 'Ser-657' of PRKCA. AKT1 regulates the balance between cell survival and apoptosis through a cascade that primarily alters the function of transcription factors that regulate pro- and antiapoptotic genes. Dephosphorylation of 'Ser-473' of AKT1 triggers apoptosis and decreases cell proliferation. Also controls the phosphorylation of AKT3. Dephosphorylation of PRKCA and PRKCB leads to their destabilization and degradation. Inhibits cancer cell proliferation and may act as a tumor suppressor.
  • 組織特異性
    In colorectal cancer tissue, expression is highest in the surface epithelium of normal colonic mucosa adjacent to the cancer tissue but is largely excluded from the crypt bases. Expression is lost or significantly decreased in 80% of tested tumors (at protein level).
  • 配列類似性
    Contains 22 LRR (leucine-rich) repeats.
    Contains 1 PH domain.
    Contains 1 PP2C-like domain.
  • 細胞内局在
    Cytoplasm. Membrane. Nucleus. In colorectal cancer tissue, expression is concentrated in the cytoplasm and nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • KIAA0931 antibody
    • PH domain and leucine rich repeat protein phosphatase 2 antibody
    • PH domain leucine rich repeat protein phosphatase like antibody
    • PH domain leucine-rich repeat-containing protein phosphatase 2 antibody
    • PH domain leucine-rich repeat-containing protein phosphatase-like antibody
    • PHLP2_HUMAN antibody
    • PHLPP like antibody
    • PHLPP-like antibody
    • Phlpp2 antibody
    • PHLPPL antibody
    see all

画像

  • Anti-PHLPP2 antibody (ab62830) at 0.1 µg/ml + Human Brain (Amygdala, Hippocampus, Substantia Nigra) lysate at 35 µg

    Predicted band size : 147 kDa
    Observed band size : 147 kDa

プロトコール

参考文献

ab62830 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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