Anti-PHLDA3 抗体 [4B6] (ab81464)
Key features and details
- Mouse monoclonal [4B6] to PHLDA3
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG2b
製品の概要
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製品名
Anti-PHLDA3 antibody [4B6]
PHLDA3 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [4B6] to PHLDA3 -
由来種
Mouse -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Synthetic peptide: MTAAATATVL KEGVLEKRSG GLLQLWKRKR C, corresponding to N terminal amino acids 1-31 of human PHLDA3
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ポジティブ・コントロール
- MDA-MB-468 cells
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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精製度
Ion Exchange Chromatography -
特記事項(精製)
The antibody was produced from the hybridoma cultured in serum free medium and purified under mild conditions by propriety chromatography processes. The antibody is filter sterilized. -
ポリ/モノ
モノクローナル -
クローン名
4B6 -
アイソタイプ
IgG2b -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab81464の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use at an assay dependent dilution. Predicted molecular weight: 14 kDa.
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特記事項 |
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WB
Use at an assay dependent dilution. Predicted molecular weight: 14 kDa. |
ターゲット情報
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機能
p53/TP53-regulated repressor of Akt/AKT1 signaling. Represses AKT1 by preventing AKT1-binding to membrane lipids, thereby inhibiting AKT1 translocation to the cellular membrane and activation. Contributes to p53/TP53-dependent apoptosis by repressing AKT1 activity. Its directs transcription regulation by p53/TP53 may explain how p53/TP53 can negatively regulate AKT1. May acts as a tumor suppressor. -
組織特異性
Widely expressed with lowest expression in liver and spleen. -
配列類似性
Belongs to the PHLDA3 family.
Contains 1 PH domain. -
ドメイン
The PH domain binds phosphoinositides with a broad specificity. It competes with the PH domain of AKT1 and directly interferes with AKT1 binding to phosphatidylinositol 4,5-bisphosphate (PIP2) and phosphatidylinositol 3,4,5-triphosphate (PIP3), preventing AKT1 association to membrane lipids and subsequent activation of AKT1 signaling. -
細胞内局在
Cytoplasm. Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 23612 Human
- Entrez Gene: 27280 Mouse
- Entrez Gene: 363989 Rat
- Omim: 607054 Human
- SwissProt: Q9Y5J5 Human
- SwissProt: Q9WV95 Mouse
- SwissProt: Q5PQT7 Rat
- Unigene: 268557 Human
see all -
別名
- PHLA3_HUMAN antibody
- PHLDA 3 antibody
- phlda3 antibody
see all
画像
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab81464 は 2 報の論文で使用されています。
- Wang P et al. Pleckstrin homology-like domain family A, member 3, a miR-19a-3p-regulated gene, suppresses tumor growth in osteosarcoma by downregulating the Akt pathway. Bioengineered 13:3993-4009 (2022). PubMed: 35112982
- Prudovsky I et al. Phosphatidylserine colocalizes with epichromatin in interphase nuclei and mitotic chromosomes. Nucleus 3:200-10 (2012). PubMed: 22555604