製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to PHKA1
  • アプリケーション
    適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit
  • 免疫原

    Recombinant protein fragment, corresponding to a region within amino acids 828 and 1032 of Human PHKA1 (NP_002628).

  • ポジティブ・コントロール
    • 293T, A431, H1299 and HeLa cell lysates for WB and Cal27 xenograft for IHC

法規制情報

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab96752 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/3000. Predicted molecular weight: 137 kDa.
IHC-P 1/100 - 1/500.

ターゲット情報

  • 機能
    Phosphorylase b kinase catalyzes the phosphorylation of serine in certain substrates, including troponin I. The alpha chain may bind calmodulin.
  • 組織特異性
    Muscle specific. Isoform 1 is predominant in vastus lateralis muscle. Isoform 2 predominates slightly in heart, and it predominates clearly in the other tissues tested.
  • パスウェイ
    Glycan biosynthesis; glycogen metabolism.
  • 関連疾患
    Glycogen storage disease 9D (GSD9D) [MIM:300559]: A metabolic disorder characterized by slowly progressive, predominantly distal muscle weakness and atrophy. Clinical features include exercise intolerance with early fatigability, pain, cramps and occasionally myoglobinuria. Note=The disease is caused by mutations affecting the gene represented in this entry.
  • 配列類似性
    Belongs to the phosphorylase b kinase regulatory chain family.
  • 翻訳後修飾
    Although the final Cys may be farnesylated, the terminal tripeptide is probably not removed, and the C-terminus is not methylated.
  • 細胞内局在
    Cell membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 5330411D17 antibody
    • 9830108K24Rik antibody
    • kinase PHKA1 antibody
    • KPB1 antibody
    • KPB1_HUMAN antibody
    • MGC132604 antibody
    • Pcyt1b antibody
    • PHKA antibody
    • PHKA1 antibody
    • Phosphorylase b kinase regulatory subunit alpha antibody
    • Phosphorylase b kinase regulatory subunit alpha, skeletal muscle isoform antibody
    • Phosphorylase kinase alpha M subunit antibody
    • phosphorylase kinase, alpha 1 (muscle) antibody
    • RP23 210E20.1 antibody
    • skeletal muscle isoform antibody
    see all

画像

  • Anti-PHKA1 antibody (ab96752) at 1/1000 dilution + HeLa whole cell lysate at 30 µg

    Predicted band size : 137 kDa
  • ab96752, at 1/500 dilution, staining PHKA1 in Cal27 xenograft tissue by Immunohistochemistry. Formalin-fixed, paraffin-embedded section.

参考文献

ab96752 has not yet been referenced specifically in any publications.

レビューと Q&A

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Heart)
Permeabilization
Yes - Tween-20
Specification
Heart
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
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投稿 Aug 29 2016

Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Reduced Denaturing (4%-12%)
Sample
Mouse Tissue lysate - whole (Skeletal Muscle)
Specification
Skeletal Muscle
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

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Verified customer

投稿 Dec 31 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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