製品の概要

  • 製品名Anti-PHIP antibody
    PHIP 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal to PHIP
  • アプリケーション適用あり: WB, Flow Cytmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant fragment, corresponding to amino acids 1599-1706 of Human PHIP

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: None
    PBS, pH 7.2
  • Concentration information loading...
  • 精製度Protein G purified
  • ポリ/モノモノクローナル
  • アイソタイプIgG2b
  • 軽鎖の種類kappa
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab57522 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 5 µg/ml. This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.
Flow Cyt Use 1µg for 106 cells. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 関連性PHIP contains two bromo domains and eight WD repeats. It may be involved in insulin signaling. It is expressed in myeloma and epidermoid carcinoma cell lines.
  • 参照データベース
  • 別名
    • DCAF14 antibody
    • DDB1 and CUL4 associated factor 14 antibody
    • FLJ20705 antibody
    • FLJ45918 antibody
    • IRS 1 PH domain binding protein antibody
    • IRS1 PH domain binding protein antibody
    • MGC90216 antibody
    • Ndrp antibody
    • Neuronal differentiation related protein antibody
    • PH interacting protein antibody
    • Pleckstrin homology domain interacting protein antibody
    • WD repeat containing protein 11 antibody
    • WD repeat protein 11 antibody
    • WDR11 antibody
    see all

Anti-PHIP antibody 画像

  • Western blot against tagged recombinant protein immunogen using ab57522 PHIP antibody at 1ug/ml. Predicted band size of immunogen is 38 kDa
  • Overlay histogram showing HeLa cells stained with ab57522 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57522, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-PHIP antibody (ab57522) 使用論文

ab57522 has not yet been referenced specifically in any publications.

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