Anti-PHGDH/Malate dehydrogenase 抗体 [4A3-1D6] (ab57030)
Key features and details
- Mouse monoclonal [4A3-1D6] to PHGDH/Malate dehydrogenase
- Suitable for: WB, ICC/IF, IHC-P, Flow Cyt, IP
- Reacts with: Human
- Isotype: IgG1
製品の概要
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製品名
Anti-PHGDH/Malate dehydrogenase antibody [4A3-1D6]
PHGDH/Malate dehydrogenase 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [4A3-1D6] to PHGDH/Malate dehydrogenase -
由来種
Mouse -
アプリケーション
適用あり: WB, ICC/IF, IHC-P, Flow Cyt, IPmore details -
種交差性
交差種: Human -
免疫原
Recombinant full length protein corresponding to Human PHGDH/Malate dehydrogenase aa 1-550.
Database link: O43175 -
特記事項
This product was changed from ascites to tissue culture supernatant on 24/1/19. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.4 -
Concentration information loading...
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精製度
Tissue culture supernatant -
特記事項(精製)
Purified from TCS. -
ポリ/モノ
モノクローナル -
クローン名
4A3-1D6 -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab57030の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 57 kDa.
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ICC/IF |
Use at an assay dependent concentration.
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IHC-P |
Use at an assay dependent concentration.
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Flow Cyt |
Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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IP |
Use at an assay dependent concentration.
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特記事項 |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 57 kDa. |
ICC/IF
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
Flow Cyt
Use at an assay dependent concentration. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
IP
Use at an assay dependent concentration. |
ターゲット情報
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パスウェイ
Amino-acid biosynthesis; L-serine biosynthesis; L-serine from 3-phospho-D-glycerate: step 1/3. -
関連疾患
Defects in PHGDH are the cause of phosphoglycerate dehydrogenase deficiency (PHGDH deficiency) [MIM:601815]. It is characterized by congenital microcephaly, psychomotor retardation, and seizures. -
配列類似性
Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. - Information by UniProt
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参照データベース
- Entrez Gene: 26227 Human
- Omim: 606879 Human
- SwissProt: O43175 Human
- Unigene: 487296 Human
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別名
- 3 PGDH antibody
- 3-PGDH antibody
- 3-phosphoglycerate dehydrogenase antibody
see all
画像
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PHGDH/Malate dehydrogenase antibody (ab57030) at 1μg/ml + Jurkat cell lysate at 25μg/lane.
This image was generated using the ascites version of the product.
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PHGDH/Malate dehydrogenase antibody (ab57030) used in immunofluorescence at 1-5μg/ml on HeLa cells.
This image was generated using the ascites version of the product.
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PHGDH/Malate dehydrogenase antibody (ab57030) used in immunohistochemistry at 5μg/ml on formalin fixed and paraffin embedded human lymph node.
This image was generated using the ascites version of the product.
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PHGDH/Malate dehydrogenase was immunoprecipitated using 0.5mg Jurkat whole cell extract, 10µg of Mouse monoclonal to PHGDH/Malate dehydrogenase and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Jurkat whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab57030.
Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/5000 dilution.
Band: 57kDa; PHGDH/Malate dehydrogenase.This image was generated using the ascites version of the product.
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Overlay histogram showing HeLa cells stained with ab57030 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab57030, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
This image was generated using the ascites version of the product.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (12)
ab57030 は 12 報の論文で使用されています。
- Naka T et al. Identification of Novel Diagnostic Markers for Malignant Pleural Mesothelioma Using a Reverse Translational Approach Based on a Rare Synchronous Tumor. Diagnostics (Basel) 12:N/A (2022). PubMed: 35204409
- Yao S et al. One carbon metabolism in human lung cancer. Transl Lung Cancer Res 10:2523-2538 (2021). PubMed: 34295659
- You MH et al. Death-Associated Protein Kinase 1 Inhibits Progression of Thyroid Cancer by Regulating Stem Cell Markers. Cells 10:N/A (2021). PubMed: 34831219
- Piga I et al. In situ Metabolic Profiling of Ovarian Cancer Tumor Xenografts: A Digital Pathology Approach. Front Oncol 10:1277 (2020). PubMed: 32974128
- Jeon MJ et al. High Phosphoglycerate Dehydrogenase Expression Induces Stemness and Aggressiveness in Thyroid Cancer. Thyroid 30:1625-1638 (2020). PubMed: 32438862