製品の概要

  • 製品名Anti-PHF20 antibody
    PHF20 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to PHF20
  • アプリケーション適用あり: IHC-P, IPmore details
    適用なし: WB
  • 種交差性
    交差種: Human
    交差が予測される動物種: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide corresponding to a region within amino acids 900-950 (PGSPKVKEYVS KKALPEEAPA RKLLDRGGEG LLSSQHQWQF NLLTHVESLQ) of Human PHF20 (NP_057520.2).

  • ポジティブ・コントロール
    • HeLa cell lysate

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab91456 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IP Use at 5-15 µg/mg of lysate.
  • 追加情報Is unsuitable for WB.
  • ターゲット情報

    • 機能Possible transcription factor.
    • 組織特異性Expressed in heart, kidney, liver, lung, pancreas, placenta, spleen and testis. Not expressed in brain, skeletal muscle, colon, ovary, prostate, small intestine and thymus. Expressed in colon and ovary cancer cell lines while it is not expressed in the respective normal tissues.
    • 配列類似性Contains 1 A.T hook DNA-binding domain.
      Contains 1 C2H2-type zinc finger.
      Contains 1 PHD-type zinc finger.
    • 細胞内局在Nucleus.
    • Information by UniProt
    • 参照データベース
    • 別名
      • C20orf104 antibody
      • GLEA2 antibody
      • Glioma-expressed antigen 2 antibody
      • HCA58 antibody
      • Hepatocellular carcinoma-associated antigen 58 antibody
      • Novel zinc finger protein antibody
      • NZF antibody
      • PHD finger protein 20 antibody
      • PHF20 antibody
      • PHF20_HUMAN antibody
      • Transcription factor TZP antibody
      • TZP antibody
      see all

    Anti-PHF20 antibody 画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung carcinoma tissue labelling PHF20 with ab91456 at 1/1000 (1µg/ml). Detection: DAB.
    • Detection of Human PHF20 by Western Blot of Immunoprecipitates.
      Samples: Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded).
      ab91456 was used for IP at 10 µg/mg lysate (lane 1), with a control IgG in lane 2. For blotting immunoprecipitates, an anti-PHF20 was used at 1 µg/ml.
      Detection: Chemiluminescence with an exposure time of 30 seconds.

    Anti-PHF20 antibody (ab91456) 使用論文

    ab91456 has not yet been referenced specifically in any publications.

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    There are currently no Abreviews or Questions for ab91456.
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