製品の概要

  • 製品名
    Anti-PHF16 antibody
    PHF16 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to PHF16
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig
  • 免疫原

    Synthetic peptide corresponding to a region within internal sequence amino acids 360-409 (SYCLKHSQNR QKLGEAEYPH HRAKEQSQAK SEKTSLRAQK LRELEEEFYS) of Human PHF16 (NP_055550).

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • ポジティブ･コントロール
    • Placenta lysate.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab85705 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 90 kDa (predicted molecular weight: 94 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 機能
    Component of the HBO1 complex which has a histone H4-specific acetyltransferase activity, a reduced activity toward histone H3 and is responsible for the bulk of histone H4 acetylation in vivo.
  • 組織特異性
    Ubiquitously expressed, with highest levels in placenta and uterus.
  • 配列類似性
    Belongs to the JADE family.
    Contains 1 PHD-type zinc finger.
  • Information by UniProt
  • 参照データベース
    • 別名
      • JADE 3 antibody
      • Jade family PHD finger protein 3 antibody
      • JADE3 antibody
      • JADE3_HUMAN antibody
      • KIAA0215 antibody
      • MGC138748 antibody
      • MGC138749 antibody
      • PHD finger protein 16 antibody
      • PHF 16 antibody
      • Phf16 antibody
      • Protein Jade 3 antibody
      • Protein Jade-3 antibody
      see all

    画像

    • Western blot - PHF16 antibody (ab85705)
      Western blot - PHF16 antibody (ab85705)
      Anti-PHF16 antibody (ab85705) at 1 µg/ml (5% skim milk / PBS buffer) + Placenta lysate at 10 µg

      Secondary
      HRP conjugated anti-Rabbit IgG at 1/50000 dilution

      Predicted band size: 94 kDa
      Observed band size: 90 kDa (why is the actual band size different from the predicted?)



      Gel concentration: 6-18%

    プロトコール

    参考文献

    ab85705 has not yet been referenced specifically in any publications.

    ab85705 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

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