Rabbit polyclonal to PHF13
Mouse, Human, Chinese hamster
Synthetic peptide conjugated to KLH, corresponding to a region within internal sequence amino acids 270-300 of Human PHF13
CHO cell line lysates and Mouse testis tissue
Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
Preservative: 0.09% Sodium Azide
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Immunogen affinity purified
Purified through a protein A column, followed by peptide affinity purification.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/500. Predicted molecular weight: 34 kDa.
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Modulates chromatin structure. Required for normal chromosome condensation during the early stages of mitosis. Required for normal chromosome separation during mitosis.
Contains 1 PHD-type zinc finger.
Subject to proteasomal degradation. Stable when bound to chromatin. The soluble form is rapidly degraded.
Nucleus. Nucleus > nucleoplasm. Predominantly bound to chromatin, but a minor proportion is also detected in the nucleoplasm.
Information by UniProt
PHD finger protein 13 antibody
Western blot - PHF13 antibody (ab103387)
Anti-PHF13 antibody (ab103387) at 1/100 dilution + CHO cell line lysates at 35 µg
Predicted band size: 34 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - PHF13 antibody (ab103387)
ab103387, at 1/50 dilution, staining PHF13 in formalin-fixed, paraffin-embedded Mouse testis tissue by Immunohistochemistry followed by peroxidase conjugation of the secondary antibody and DAB detection.
has not yet been referenced specifically in any publications.
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