製品の概要

  • 製品名
  • 製品の詳細
    Mouse polyclonal to PHF10
  • アプリケーション
    適用あり: ICC/IF, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Full length protein, corresponding to amino acids 1-410 of Human PHF10

  • ポジティブ・コントロール
    • HeLa cells

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • 精製度
    Protein A purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab68114 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 10 µg/ml.
WB 1/500 - 1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 47 kDa). This antibody has only been tested against a recombinant cell lysate.

ターゲット情報

  • 機能
    Involved in transcription activity regulation by chromatin remodeling. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and is required for the proliferation of neural progenitors. During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth.
  • 配列類似性
    Belongs to the SAYP family.
    Contains 2 PHD-type zinc fingers.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • BAF45a antibody
    • BRG1-associated factor 45a antibody
    • FLJ10975 antibody
    • MGC111009 antibody
    • PHD finger protein 10 antibody
    • PHD finger protein 10, isoform a antibody
    • PHD zinc finger protein XAP135 antibody
    • Phf10 antibody
    • PHF10_HUMAN antibody
    • XAP135 antibody
    see all

画像

  • All lanes : Anti-PHF10 antibody (ab68114) at 1/500 dilution

    Lane 1 : PHF10 transfected 293T cell lysate
    Lane 2 : Non transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugated at 1/2500 dilution

    Predicted band size : 47 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
  • Immunofluorescence analysis of HeLa cells labelled with purified ab68114 at 10 µg/ml followed by fluorescein-conjugated secondary antibody. Cells were fixed in 4% paraformaldehyde and permeabilised with 0.1% Triton X-100.

参考文献

ab68114 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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