Rabbit polyclonal to PHF1
Mouse, Rat, Cow, Cynomolgus monkey, Macaque monkey
Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human PHF1.
(Peptide available as
This antibody gave a positive signal in Human Heart Tissue Lysate.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa).
Use a concentration of 1 µg/ml.
Transcriptional repressor. May promote methylation of histone H3 on 'Lys-27' by the PRC2/EED-EZH2 complex.
Highest levels in heart, skeletal muscle, and pancreas, lower levels in brain, placenta, lung, liver and kidney.
Contains 2 PHD-type zinc fingers.
Nucleus. Localizes specifically to the promoters of numerous target genes.
Information by UniProt
MTF2 L2 antibody
MTF2L 2 antibody
Western blot - Anti-PHF1 antibody (ab80042)
Anti-PHF1 antibody (ab80042) at 1 µg/ml + Human heart tissue lysate - total protein (
) at 10 µg
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size:
Observed band size:
Additional bands at:
50 kDa (possible isoform)
This antibody is predicted to cross react with both isoform 1 (50-kDa) and isoform 2 (62-kDa) of PHF1. We believe that the band observed at 50-kDa corresponds to isoform 1.
Immunocytochemistry/ Immunofluorescence - Anti-PHF1 antibody (ab80042)
ICC/IF image of ab80042 stained MCF-7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab80042, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% Methanol fixed (5 min) HeLa cells at 1µg/ml.
has not yet been referenced specifically in any publications.
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