製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to PHF1
  • アプリケーション
    適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Cow, Cynomolgus monkey, Macaque monkey
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human PHF1.

    (Peptide available as ab88938.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in Human Heart Tissue Lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab80042 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 62 kDa (predicted molecular weight: 62 kDa).
ICC/IF Use a concentration of 1 µg/ml.

ターゲット情報

  • 機能
    Transcriptional repressor. May promote methylation of histone H3 on 'Lys-27' by the PRC2/EED-EZH2 complex.
  • 組織特異性
    Highest levels in heart, skeletal muscle, and pancreas, lower levels in brain, placenta, lung, liver and kidney.
  • 配列類似性
    Contains 2 PHD-type zinc fingers.
  • 細胞内局在
    Nucleus. Localizes specifically to the promoters of numerous target genes.
  • Information by UniProt
  • 参照データベース
  • 別名
    • hPCl1 antibody
    • MTF2 L2 antibody
    • MTF2L 2 antibody
    • MTF2L2 antibody
    • PCL 1 antibody
    • PCL1 antibody
    • PHD finger protein 1 antibody
    • PHD finger protein1 antibody
    • PHF 1 antibody
    • PHF 2 antibody
    • Phf1 antibody
    • PHF1_HUMAN antibody
    • PHF2 antibody
    • Plc 1 antibody
    • Plc1 antibody
    • Polycomb like protein 1 antibody
    • Polycomb like protein1 antibody
    • Polycomb-like protein 1 antibody
    • Protein PHF1 antibody
    • T complex testis expressed 3 antibody
    • T complex testis expressed3 antibody
    • Tctex 3 antibody
    • Tctex3 antibody
    see all

画像

  • Anti-PHF1 antibody (ab80042) at 1 µg/ml + Human heart tissue lysate - total protein (ab29431) at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 62 kDa
    Observed band size : 62 kDa
    Additional bands at : 50 kDa (possible isoform).

    Exposure time : 3 minutes
  • ICC/IF image of ab80042 stained MCF-7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab80042, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% Methanol fixed (5 min) HeLa cells at 1µg/ml.

参考文献

ab80042 has not yet been referenced specifically in any publications.

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