Anti-PHD2 / prolyl hydroxylase 抗体 [AT1E9] (ab100840)


  • 製品名Anti-PHD2 / prolyl hydroxylase antibody [AT1E9]
    PHD2 / prolyl hydroxylase 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [AT1E9] to PHD2 / prolyl hydroxylase
  • アプリケーション適用あり: WB, ELISA, ICC/IFmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant fragment, corresponding to amino acids 271-426 of Human PHD2/ prolyl hydroxylase purified from E.coli (NP_071334).

  • ポジティブ・コントロール
    • HeLa cell lysate, HeLa cells



Our Abpromise guarantee covers the use of ab100840 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Predicted molecular weight: 46 kDa.
ELISA Use at an assay dependent dilution.
ICC/IF 1/500 - 1/1000.


  • 機能Catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates HIF-1 alpha at 'Pro-402' and 'Pro-564', and HIF-2 alpha. Functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex.
  • 組織特異性According to PubMed:11056053, widely expressed with highest levels in skeletal muscle and heart, moderate levels in pancreas, brain (dopaminergic neurons of adult and fetal substantia nigra) and kidney, and lower levels in lung and liver. According to PubMed:12351678 widely expressed with highest levels in brain, kidney and adrenal gland. Expressed in cardiac myocytes, aortic endothelial cells and coronary artery smooth muscle.
  • 関連疾患Defects in EGLN1 are the cause of erythrocytosis familial type 3 (ECYT3) [MIM:609820]. ECYT3 is an autosomal dominant disorder characterized by increased serum red blood cell mass, elevated serum hemoglobin and hematocrit, and normal serum erythropoietin levels.
  • 配列類似性Contains 1 Fe2OG dioxygenase domain.
    Contains 1 MYND-type zinc finger.
  • Information by UniProt
  • 参照データベース
  • 別名
    • C1ORF12 antibody
    • Chromosome 1 Open Reading Frame 12 antibody
    • DKFZp761F179 antibody
    • ECYT 3 antibody
    • ECYT3 antibody
    • EGL 9 homolog of C. elegans 1 antibody
    • EGL nine (C.elegans) homolog 1 antibody
    • Egl nine homolog 1 (C. elegans) antibody
    • Egl nine homolog 1 antibody
    • Egl nine like protein 1 antibody
    • EGLN 1 antibody
    • Egln1 antibody
    • EGLN1_HUMAN antibody
    • HIF PH2 antibody
    • HIF Prolyl Hydroxylase 2 antibody
    • HIF-PH2 antibody
    • HIF-prolyl hydroxylase 2 antibody
    • HIFP4H 2 antibody
    • HIFPH2 antibody
    • HPH 2 antibody
    • HPH-2 antibody
    • HPH2 antibody
    • Hypoxia inducible factor prolyl hydroxylase 2 antibody
    • Hypoxia-inducible factor prolyl hydroxylase 2 antibody
    • ORF13 antibody
    • P4H2 antibody
    • PHD 2 antibody
    • PhD2 antibody
    • PNAS 118 antibody
    • PNAS 137 antibody
    • Prolyl Hydroxylase Domain Containing Protein 2 antibody
    • Prolyl hydroxylase domain-containing protein 2 antibody
    • Rat Homolog of SM20 antibody
    • SM 20 antibody
    • SM-20 antibody
    • SM20 antibody
    • Zinc finger MYND domain containing protein 6 antibody
    • ZMYND6 antibody
    see all

Anti-PHD2 / prolyl hydroxylase antibody [AT1E9] 画像

  • Anti-PHD2 / prolyl hydroxylase antibody [AT1E9] (ab100840) at 1/1000 dilution + HeLa cell lysate at 35 µg

    HRP conjugated goat anti-mouse
    developed using the ECL technique

    Predicted band size : 46 kDa
  • Immunofluorescence analysis of HeLa cells
    Top right: PHD2 / prolyl hydroxylase stained with ab100840 at 1/500 dilution
    Top left: Actin stained by Phalloidin-TRITC
    Bottom left: Nucleus stained by Hoechst 33342
    Bottom right: all 3 images merged

Anti-PHD2 / prolyl hydroxylase antibody [AT1E9] (ab100840) 使用論文

ab100840 has not yet been referenced specifically in any publications.

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