Anti-PHD1/prolyl hydroxylase 抗体 [EPR2746] (ab113077)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2746] to PHD1/prolyl hydroxylase
- Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-PHD1/prolyl hydroxylase antibody [EPR2746]
PHD1/prolyl hydroxylase 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR2746] to PHD1/prolyl hydroxylase -
由来種
Rabbit -
アプリケーション
適用あり: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: A549, HeLa and MCF-7 cell lysates, Human fetal heart, Mouse brain, Mouse heart, Rat brain, Rat heart tissue lysates IHC-P: Mouse testis, Rat spleen, Human breast carcinoma and Human lung carcinoma tissue ICC/IF: HeLa cells. Flow Cyt (intra): HeLa cells.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR2746 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab113077の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Flow Cyt (Intra) |
1/30.
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WB | (2) |
1/1000 - 1/10000. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa).
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IHC-P |
1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
See IHC antigen retrieval protocols. For unpurified use at 1/100 - 1/250. |
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ICC/IF |
1/50 - 1/100.
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特記事項 |
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Flow Cyt (Intra)
1/30. |
WB
1/1000 - 1/10000. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa). |
IHC-P
1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. See IHC antigen retrieval protocols. For unpurified use at 1/100 - 1/250. |
ICC/IF
1/50 - 1/100. |
ターゲット情報
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機能
Catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates HIF-1 alpha at 'Pro-402' and 'Pro-564', and HIF-2 alpha. Functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. May play a role in cell growth regulation. Isoform p40 and isoform p43 exhibit the same level of activity. -
組織特異性
Expressed abundantly in all tissues with highest expression in testis. Expressed in hormone responsive tissues, including normal and cancerous mammary, ovarian and prostate epithelium. -
配列類似性
Contains 1 Fe2OG dioxygenase domain. -
細胞内局在
Cytoplasm. Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 112398 Human
- Entrez Gene: 112406 Mouse
- Entrez Gene: 308457 Rat
- Omim: 606424 Human
- SwissProt: Q96KS0 Human
- SwissProt: Q91YE2 Mouse
- SwissProt: Q6AYU4 Rat
- Unigene: 515417 Human
see all -
別名
- DKFZp434E026 antibody
- EGL nine (C.elegans) homolog 2 antibody
- Egl nine homolog 2 (C. elegans) antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat spleen tissue sections labeling PHD1/prolyl hydroxylase with Purified ab113077 at 1:50 dilution (5.14 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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Immunocytochemistry/ Immunofluorescence analysis of HeLa (human cervix adenocarcinoma epithelial cell) cells labeling PHD1/prolyl hydroxylase with purified ab113077 at 1/50 dilution (5 µg/mL). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/mL) dilution. DAPI (blue) was used as nuclear counterstain. ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 µg/mL) was used as the secondary antibody only control.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse testis tissue sections labeling PHD1/prolyl hydroxylase with Purified ab113077 at 1:50 dilution (5.14 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human breast cancer tissue sections labeling PHD1/prolyl hydroxylase with Purified ab113077 at 1:50 dilution (5.14 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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Intracellular Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling PHD1/prolyl hydroxylase with Purified ab113077 at 1/30 dilution (10µg/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluorr® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
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All lanes : Anti-PHD1/prolyl hydroxylase antibody [EPR2746] (ab113077) at 1/1000 dilution (Purified)
Lane 1 : MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : Human fetal heart lysates
Lane 3 : Mouse brain lysates
Lane 4 : Mouse heart lysates
Lane 5 : Rat brain lysates
Lane 6 : Rat heart lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 44 kDa
Observed band size: 40,43 kDa why is the actual band size different from the predicted? -
All lanes : Anti-PHD1/prolyl hydroxylase antibody [EPR2746] (ab113077) at 1/1000 dilution
Lane 1 : A549 cell lysates
Lane 2 : HeLa cell lysates
Lane 3 : MCF-7 cell lysates
Lysates/proteins at 10 µg per lane.
Predicted band size: 44 kDaThis image was generated using the unpurified version of the product.
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ab113077, at a 1/100 dilution, staining PHD1/prolyl hydroxylase in paraffin-embedded Human breast carcinoma tissue by Immunohistochemistry.
This image was generated using the unpurified version of the product.
Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
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ab113077, at a 1/100 dilution, staining PHD1/prolyl hydroxylase in paraffin-embedded Human lung carcinoma tissue by Immunohistochemistry.
This image was generated using the unpurified version of the product.
Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab113077 は 5 報の論文で使用されています。
- Sato T et al. Enhanced glucose metabolism through activation of HIF-1α covers the energy demand in a rat embryonic heart primordium after heartbeat initiation. Sci Rep 12:74 (2022). PubMed: 34996938
- Liu X et al. Histone H3 proline 16 hydroxylation regulates mammalian gene expression. Nat Genet 54:1721-1735 (2022). PubMed: 36347944
- Ju C et al. Hypoxia-inducible factor-1α-dependent induction of miR122 enhances hepatic ischemia tolerance. J Clin Invest 131:N/A (2021). PubMed: 33792566
- Tsai YM et al. Loss of miR-145-5p Causes Ceruloplasmin Interference with PHD-Iron Axis and HIF-2a Stabilization in Lung Adenocarcinoma-Mediated Angiogenesis. Int J Mol Sci 21:N/A (2020). PubMed: 32708433
- Zurlo G et al. Prolyl hydroxylase substrate adenylosuccinate lyase is an oncogenic driver in triple negative breast cancer. Nat Commun 10:5177 (2019). PubMed: 31729379