リコンビナント
RabMAb

Anti-PHD1/prolyl hydroxylase 抗体 [EPR2745] (ab108980)

製品の概要

  • 製品名
    Anti-PHD1/prolyl hydroxylase antibody [EPR2745]
    PHD1/prolyl hydroxylase 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR2745] to PHD1/prolyl hydroxylase
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: WB, IHC-P, Flow Cyt, ICC/IFmore details
    適用なし: IP
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide within Human PHD1/prolyl hydroxylase aa 1-100. The exact sequence is proprietary.

  • ポジティブ・コントロール
    • A549,HeLa, and MCF-7 cell lysates Human ovarian adenocarcinoma tissue Human testis tissue
  • 特記事項

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab108980 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Predicted molecular weight: 44 kDa.
IHC-P 1/100 - 1/250. Perform antigen retrieval
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

ICC/IF 1/100 - 1/250.
  • 追加情報
    Is unsuitable for IP.
  • ターゲット情報

    • 機能
      Catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates HIF-1 alpha at 'Pro-402' and 'Pro-564', and HIF-2 alpha. Functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. May play a role in cell growth regulation. Isoform p40 and isoform p43 exhibit the same level of activity.
    • 組織特異性
      Expressed abundantly in all tissues with highest expression in testis. Expressed in hormone responsive tissues, including normal and cancerous mammary, ovarian and prostate epithelium.
    • 配列類似性
      Contains 1 Fe2OG dioxygenase domain.
    • 細胞内局在
      Cytoplasm. Nucleus.
    • Information by UniProt
    • 参照データベース
    • 別名
      • DKFZp434E026 antibody
      • EGL nine (C.elegans) homolog 2 antibody
      • Egl nine homolog 2 (C. elegans) antibody
      • Egl nine homolog 2 antibody
      • EGLN 2 antibody
      • EGLN2 antibody
      • EGLN2_HUMAN antibody
      • EIT 6 antibody
      • EIT6 antibody
      • Estrogen-induced tag 6 antibody
      • HIF P4H 1 antibody
      • HIF PH1 antibody
      • HIF prolyl hydroxylase 1 antibody
      • HIF-PH1 antibody
      • HIF-prolyl hydroxylase 1 antibody
      • HIFPH 1 antibody
      • HIFPH1 antibody
      • HPH 3 antibody
      • HPH-1 antibody
      • HPH-3 antibody
      • HPH3 antibody
      • Hypoxia inducible factor prolyl hydroxylase 1 antibody
      • Hypoxia-inducible factor prolyl hydroxylase 1 antibody
      • P4H1 antibody
      • PHD 1 antibody
      • PhD1 antibody
      • prolyl hydroxylase domain containing protein 1 antibody
      • Prolyl hydroxylase domain-containing protein 1 antibody
      see all

    画像

    • All lanes : Anti-PHD1/prolyl hydroxylase antibody [EPR2745] (ab108980) at 1/1000 dilution

      Lane 1 : A549 cell lysates
      Lane 2 : HeLa cell lysates
      Lane 3 : MCF-7 cell lysates

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Standard HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 44 kDa

    • Immunohistochemical analysis of paraffin-embedded Human ovarian adenocarcinoma tissue using ab108980
    • Immunohistochemical analysis of paraffin-embedded Human testis tissue using ab108980
    • Immunofluorescent staining of HeLa cells using ab108980
    • Overlay histogram showing HeLa cells stained with ab108980 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab108980, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    参考文献

    This product has been referenced in:
    • Xiang J  et al. TCF7L2 positively regulates aerobic glycolysis via the EGLN2/HIF-1a axis and indicates prognosis in pancreatic cancer. Cell Death Dis 9:321 (2018). Read more (PubMed: 29476053) »
    • Liu J  et al. Double Knockdown of PHD1 and Keap1 Attenuated Hypoxia-Induced Injuries in Hepatocytes. Front Physiol 8:291 (2017). WB, IHC-P . Read more (PubMed: 28539891) »

    See all 7 Publications for this product

    レビューと Q&A

    Application
    Western blot
    Loading amount
    20 µg
    Gel Running Conditions
    Reduced Denaturing
    Sample
    Human Cell lysate - whole cell (osteosarcoma cells)
    Specification
    osteosarcoma cells
    Treatment
    control and siRNA for PHD1 and GFP-PHD1 overexpression
    Blocking step
    Milk as blocking agent for 10 minute(s) · Concentration: 10% · Temperature: 21°C
    Username

    Dr. Sonia Rocha

    Verified customer

    投稿 Aug 14 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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