製品の概要

  • 製品名
    Anti-PHD1/prolyl hydroxylase antibody
    PHD1/prolyl hydroxylase 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to PHD1/prolyl hydroxylase
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human PHD1/prolyl hydroxylase.

    (Peptide available as ab91085.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in MDA-MB231 whole cell lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab80361 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 44 kDa).

ターゲット情報

  • 機能
    Catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates HIF-1 alpha at 'Pro-402' and 'Pro-564', and HIF-2 alpha. Functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. May play a role in cell growth regulation. Isoform p40 and isoform p43 exhibit the same level of activity.
  • 組織特異性
    Expressed abundantly in all tissues with highest expression in testis. Expressed in hormone responsive tissues, including normal and cancerous mammary, ovarian and prostate epithelium.
  • 配列類似性
    Contains 1 Fe2OG dioxygenase domain.
  • 細胞内局在
    Cytoplasm. Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • DKFZp434E026 antibody
    • EGL nine (C.elegans) homolog 2 antibody
    • Egl nine homolog 2 (C. elegans) antibody
    • Egl nine homolog 2 antibody
    • EGLN 2 antibody
    • EGLN2 antibody
    • EGLN2_HUMAN antibody
    • EIT 6 antibody
    • EIT6 antibody
    • Estrogen-induced tag 6 antibody
    • HIF P4H 1 antibody
    • HIF PH1 antibody
    • HIF prolyl hydroxylase 1 antibody
    • HIF-PH1 antibody
    • HIF-prolyl hydroxylase 1 antibody
    • HIFPH 1 antibody
    • HIFPH1 antibody
    • HPH 3 antibody
    • HPH-1 antibody
    • HPH-3 antibody
    • HPH3 antibody
    • Hypoxia inducible factor prolyl hydroxylase 1 antibody
    • Hypoxia-inducible factor prolyl hydroxylase 1 antibody
    • P4H1 antibody
    • PHD 1 antibody
    • PhD1 antibody
    • prolyl hydroxylase domain containing protein 1 antibody
    • Prolyl hydroxylase domain-containing protein 1 antibody
    see all

画像

  • Anti-PHD1/prolyl hydroxylase antibody (ab80361) at 1 µg/ml + MDA-MB-231 (Human breast adenocarcinoma cell line) Whole Cell Lysate at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Predicted band size : 44 kDa
    Observed band size : 45 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 100 kDa,37 kDa,55 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes
  • ICC/IF image of ab80361 stained HeLa cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab80361, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in HepG2, Hek293 and MCF7 cells. However, this Fast-Track antibody is not yet fully characterised. This image represents inconclusive preliminary data.

プロトコール

参考文献

This product has been referenced in:
  • Chan MC  et al. Tuning the Transcriptional Response to Hypoxia by Inhibiting Hypoxia-inducible Factor (HIF) Prolyl and Asparaginyl Hydroxylases. J Biol Chem 291:20661-73 (2016). Human . Read more (PubMed: 27502280) »

See 1 Publication for this product

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