製品の概要

  • 製品名Anti-PHD1/prolyl hydroxylase antibody
    PHD1/prolyl hydroxylase 一次抗体 製品一覧
  • 製品の詳細
    Mouse polyclonal to PHD1/prolyl hydroxylase
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Caenorhabditis elegans
  • 免疫原

    Recombinant fusion protein: RNPHEVMPVF RHRFAITIWY MDKSERDKAL AKGKESDAAC, corresponding to amino acids 545-644 of Caenorhabditis elegans PHD1

  • 特記事項


    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab52726 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Detects a band of approximately 38 kDa (predicted molecular weight: 44 kDa).

This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.

ターゲット情報

  • 機能Catalyzes the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. Hydroxylates HIF-1 alpha at 'Pro-402' and 'Pro-564', and HIF-2 alpha. Functions as a cellular oxygen sensor and, under normoxic conditions, targets HIF through the hydroxylation for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. May play a role in cell growth regulation. Isoform p40 and isoform p43 exhibit the same level of activity.
  • 組織特異性Expressed abundantly in all tissues with highest expression in testis. Expressed in hormone responsive tissues, including normal and cancerous mammary, ovarian and prostate epithelium.
  • 配列類似性Contains 1 Fe2OG dioxygenase domain.
  • 細胞内局在Cytoplasm. Nucleus.
  • Information by UniProt
  • 別名
    • DKFZp434E026 antibody
    • EGL nine (C.elegans) homolog 2 antibody
    • Egl nine homolog 2 (C. elegans) antibody
    • Egl nine homolog 2 antibody
    • EGLN 2 antibody
    • EGLN2 antibody
    • EGLN2_HUMAN antibody
    • EIT 6 antibody
    • EIT6 antibody
    • Estrogen-induced tag 6 antibody
    • HIF P4H 1 antibody
    • HIF PH1 antibody
    • HIF prolyl hydroxylase 1 antibody
    • HIF-PH1 antibody
    • HIF-prolyl hydroxylase 1 antibody
    • HIFPH 1 antibody
    • HIFPH1 antibody
    • HPH 3 antibody
    • HPH-1 antibody
    • HPH-3 antibody
    • HPH3 antibody
    • Hypoxia inducible factor prolyl hydroxylase 1 antibody
    • Hypoxia-inducible factor prolyl hydroxylase 1 antibody
    • P4H1 antibody
    • PHD 1 antibody
    • PhD1 antibody
    • prolyl hydroxylase domain containing protein 1 antibody
    • Prolyl hydroxylase domain-containing protein 1 antibody
    see all

Anti-PHD1/prolyl hydroxylase antibody 画像

  • All lanes : Anti-PHD1/prolyl hydroxylase antibody (ab52726) at 1/1000 dilution

    Lane 1 : Total protein extract from E. coli with ~50ng to 100ng of a recombinant fusion protein of an irrelevant antigen
    Lane 2 : Total protein extract from E. coli with ~50ng to 500ng of the antigen (tagged recombinant fusion protein)

    Secondary
    Rabbit anti-mouse IgG + IgM, horseradish peroxidase conjugated at 1/5000 dilution

    Predicted band size : 44 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)

Anti-PHD1/prolyl hydroxylase antibody (ab52726) 使用論文

This product has been referenced in:

See 1 Publication for this product

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