製品の概要

  • 製品名Anti-PHD finger protein 6 antibody
    PHD finger protein 6 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to PHD finger protein 6
  • アプリケーション適用あり: WB, IP, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Chinese Hamster, Orangutan, Elephant
  • 免疫原

    Synthetic peptide, corresponding to a region between amino acids 315-365 of Human PHD finger protein 6 (NP_115834.1).

  • ポジティブ・コントロール
    • HeLa and 293T whole cell lysates.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)ab99395 was affinity purified using an epitope specific to PHD finger protein 6 immobilized on solid support.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab99395 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Predicted molecular weight: 41 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能May play a role in transcriptional regulation.
  • 組織特異性Ubiquitously expressed.
  • 関連疾患Defects in PHF6 are the cause of Boerjeson-Forssman-Lehmann syndrome (BFLS) [MIM:301900]; also known as Boerjeson-Forssman syndrome (BORJ). BFLS is a X-linked recessive disorder characterized by moderate to severe mental retardation, epilepsy, hypogonadism, hypometabolism, obesity with marked gynecomastia, swelling of subcutaneous tissue of the face, narrow palpebral fissure and large but not deformed ears.
  • 配列類似性Contains 2 PHD-type zinc fingers.
  • 翻訳後修飾Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 細胞内局在Nucleus. Nucleus > nucleolus. Nuclear, it particularly localizes to the nucleolus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AC004383.6 antibody
    • BFLS antibody
    • BORJ antibody
    • CENP 31 antibody
    • Centromere protein 31 antibody
    • MGC14797 antibody
    • OTTHUMP00000024063 antibody
    • PHD finger protein 6 antibody
    • PHD like zinc finger protein antibody
    • PHD-like zinc finger protein antibody
    • Phf6 antibody
    • PHF6_HUMAN antibody
    see all

Anti-PHD finger protein 6 antibody 画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skin carcinoma tissue labelling PHD finger protein 6 with ab99395 at 1/200 (1µg/ml). Detection: DAB.
  • All lanes : Anti-PHD finger protein 6 antibody (ab99395) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysates at 50 µg
    Lane 2 : HeLa whole cell lysates at 15 µg
    Lane 3 : HeLa whole cell lysates at 5 µg
    Lane 4 : 293T whole cell lysates at 50 µg

    Developed using the ECL technique

    Predicted band size : 41 kDa


    Exposure time : 3 seconds
  • Detection of PHD finger protein 6 by Western Blot of Immunprecipitate.
    ab99395 at 1µg/ml staining PHD finger protein 6 in HeLa whole cell lysate immunoprecipitated using ab99395 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 10 seconds.

Anti-PHD finger protein 6 antibody (ab99395) 使用論文

ab99395 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab99395.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"