Anti-PGRPS 抗体 [188C424] (ab13903)
Key features and details
- Mouse monoclonal [188C424] to PGRPS
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG1
製品の概要
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製品名
Anti-PGRPS antibody [188C424]
PGRPS 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [188C424] to PGRPS -
由来種
Mouse -
アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide: YVLKGHRDVQRTLSPG , corresponding to amino acids 165-180 of Human PGRPS.
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ポジティブ・コントロール
- IHC: Human esophagus cells. WB: Jurkat cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.05% Sodium azide
Constituents: PBS, 0.05% BSA -
Concentration information loading...
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精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
188C424 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab13903の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
Use a concentration of 20 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
Use a concentration of 2 µg/ml. Predicted molecular weight: 22 kDa.
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特記事項 |
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IHC-P
Use a concentration of 20 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use a concentration of 2 µg/ml. Predicted molecular weight: 22 kDa. |
ターゲット情報
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機能
Pattern receptor that binds to murein peptidoglycans (PGN) of Gram-positive bacteria. Has bactericidal activity towards Gram-positive bacteria. May kill Gram-positive bacteria by interfering with peptidoglycan biosynthesis. Binds also to Gram-negative bacteria, and has bacteriostatic activity towards Gram-negative bacteria. Plays a role in innate immunity. -
組織特異性
Highly expressed in bone marrow. Weak expression found in kidney, liver, small intestine, spleen, thymus, peripheral leukocyte, lung, fetal spleen and neutrophils. -
配列類似性
Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family. -
翻訳後修飾
N-glycosylated. N-glycosylation is required for bactericidal activity. -
細胞内局在
Secreted. Cytoplasmic granule. - Information by UniProt
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参照データベース
- Entrez Gene: 8993 Human
- Omim: 604963 Human
- SwissProt: O75594 Human
- Unigene: 137583 Human
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別名
- MGC126894 antibody
- MGC126896 antibody
- Peptidoglycan recognition protein 1 antibody
see all
画像
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Immunocytochemistry analysis of human esophagus cells labelling PGRPS with ab13903 at 20 µg/mL. The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Staining of peripheral leukocytes and neutrophils was observed. Staining was performed by Histowiz.
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Anti-PGRPS antibody [188C424] (ab13903) at 2 µg/ml + Jurkat cell lysate
Predicted band size: 22 kDa
Observed band size: 28 kDa why is the actual band size different from the predicted?
データシートおよび資料
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Datasheet download
参考文献 (1)
ab13903 は 1 報の論文で使用されています。
- Dey S & Bishayi B Riboflavin along with antibiotics balances reactive oxygen species and inflammatory cytokines and controls Staphylococcus aureus infection by boosting murine macrophage function and regulates inflammation. J Inflamm (Lond) 13:36 (2016). IF . PubMed: 27932936