Rabbit polyclonal to PGRMC2
Synthetic peptide, corresponding to a region between amino acids 1-50 of Human PGRMC2 (NP_006311.1).
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Constituents: Tris citrate/phosphate, pH 7 to 8
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Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at 2-5 µg/mg of lysate.
Is unsuitable for WB.
Membrane associated progesterone receptor component 2 (PGRMC2) belongs to the cytochrome b5 family. The molecular function is primarily steroid binding.
Membrane associated progesterone receptor component 2 antibody
Immunoprecipitation - Anti-PGRMC2 antibody (ab104437)
Detection of PGRMC2 in immunoprecipitates of HeLa whole cell lysate (1 mg lysate for IP, 20% of IP loaded on gel) using ab104437 at 3 µg/mg lysate for IP. An anti-PGRMC2 antibody which recognizes a downstream epitope was used at 1 µg/ml for subsequent western blot detection.
Detection: chemiluminescence with exposure time of 10 seconds.
has not yet been referenced specifically in any publications.
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