製品の概要

  • 製品名
  • 製品の詳細
    Goat polyclonal to PGRMC1
  • アプリケーション
    適用あり: ICC/IF, WB, IHC-Pmore details
  • 種交差性
    交差種: Human, Macaque monkey
    交差が予測される動物種: Mouse, Rat, Cow, Pig
  • 免疫原

    Synthetic peptide:

    C-EPKDESARKND

    , corresponding to C terminal amino acids 185-195 of Human PGRMC1.

  • ポジティブ・コントロール
    • Human kidney, liver and testis lysates.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab48012 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 0.1 - 0.33 µg/ml. Detects a band of approximately 25 kDa (predicted molecular weight: 22 kDa).
IHC-P Use at an assay dependent concentration. PubMed: 22307145

ターゲット情報

Anti-PGRMC1 antibody 画像

  • Anti-PGRMC1 antibody (ab48012) at 0.1 µg/ml + Human Kidney lysate in RIPA buffer at 35 µg
    Developed using the ECL technique

    Predicted band size : 22 kDa
    Observed band size : 25 kDa (why is the actual band size different from the predicted?)
  • ICC/IF image of ab48012 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab48012, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 donkey anti-goat IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-PGRMC1 antibody (ab48012) 使用論文

This product has been referenced in:
  • Zhang D  et al. PGRMC1 Is a Novel Potential Tumor Biomarker of Human Renal Cell Carcinoma Based on Quantitative Proteomic and Integrative Biological Assessments. PLoS One 12:e0170453 (2017). WB, IHC . Read more (PubMed: 28107520) »
  • Peluso JJ  et al. Evidence for a genomic mechanism of action for progesterone receptor membrane component-1. Steroids 77:1007-12 (2012). WB ; Human . Read more (PubMed: 22326699) »

See all 8 Publications for this product

Product Wall

Application
Immunohistochemistry free floating
Sample
Mouse Tissue sections (Brain)
Specification
Brain
Username

Miss. Trinh Nguyen

Verified customer

投稿 Sep 07 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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