Anti-PGP9.5 抗体 - Neuronal Marker (ab15503)
Key features and details
- Rabbit polyclonal to PGP9.5 - Neuronal Marker
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-PGP9.5 antibody - Neuronal Marker
PGP9.5 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PGP9.5 - Neuronal Marker -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IF, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Recombinant full length protein corresponding to Human PGP9.5.
Database link: P09936 -
ポジティブ・コントロール
- Pancreas and small intestine This antibody gave a positive result when used in the following formaldehyde fixed cell lines: DU145.
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特記事項
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.60
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab15503の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (1) |
Use at an assay dependent concentration. Predicted molecular weight: 25 kDa.
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ICC/IF | (1) |
Use a concentration of 1 µg/ml.
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IHC-P | (3) |
1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Cool at RT for 20 minutes.
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特記事項 |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 25 kDa. |
ICC/IF
Use a concentration of 1 µg/ml. |
IHC-P
1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Cool at RT for 20 minutes. |
ターゲット情報
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機能
Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity. -
組織特異性
Found in neuronal cell bodies and processes throughout the neocortex (at protein level). Expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. Weakly expressed in ovary. Down-regulated in brains from Parkinson disease and Alzheimer disease patients. -
関連疾患
Parkinson disease 5
Neurodegeneration with optic atrophy, childhood-onset -
配列類似性
Belongs to the peptidase C12 family. -
翻訳後修飾
O-glycosylated. -
細胞内局在
Cytoplasm. Endoplasmic reticulum membrane. About 30% of total UCHL1 is associated with membranes in brain. - Information by UniProt
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参照データベース
- Entrez Gene: 7345 Human
- Omim: 191342 Human
- SwissProt: P09936 Human
- Unigene: 518731 Human
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別名
- Epididymis luminal protein 117 antibody
- Epididymis secretory protein Li 53 antibody
- HEL 117 antibody
see all
画像
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Formalin-fixed, paraffin-embedded human pancreas tissue stained for PGP9.5 using ab15503 at 1/200 dilution in immunohistochemical analysis.
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ICC/IF image of ab15503 stained DU145. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab15503 at 1 ug/mL overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Anti-PGP9.5 antibody - Neuronal Marker (ab15503) at 1/25 dilution + Human Brain lysate
Predicted band size: 25 kDa
Observed band size: 26 kDa why is the actual band size different from the predicted?
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (17)
ab15503 は 17 報の論文で使用されています。
- Astono IP et al. Objective quantification of nerves in immunohistochemistry specimens of thyroid cancer utilising deep learning. PLoS Comput Biol 18:e1009912 (2022). PubMed: 35226665
- Albayrak M et al. Prognostic value of cutaneous reinnervation with GAP-43 in oxaliplatin-induced neuropathy. J Neurol 269:4174-4184 (2022). PubMed: 35258850
- Semaniakou A et al. Disrupted local innervation results in less VIP expression in CF mice tissues. J Cyst Fibros 20:154-164 (2021). PubMed: 32600901
- Ferdoushi A et al. Tumor innervation and clinical outcome in pancreatic cancer. Sci Rep 11:7390 (2021). PubMed: 33795769
- Joyce JD et al. Assessment of Two Novel Live-Attenuated Vaccine Candidates for Herpes Simplex Virus 2 (HSV-2) in Guinea Pigs. Vaccines (Basel) 9:N/A (2021). PubMed: 33805768