Anti-PGP9.5 抗体 - Neuronal Marker (ab10410)


  • 製品名Anti-PGP9.5 antibody - Neuronal Marker
    PGP9.5 一次抗体 製品一覧
  • 製品の詳細
    Guinea pig polyclonal to PGP9.5 - Neuronal Marker
  • アプリケーション適用あり: IHC-Fr, IHC-P, IHC-FoFr, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat, Human
    交差が予測される動物種: Macaque Monkey
  • 免疫原

    Synthetic peptide:


    , corresponding to amino acids 175-191 of soluble cytoplasmic protein PGP9.5, human.

  • ポジティブ・コントロール
    • IF: DU145 cell line



Our Abpromise guarantee covers the use of ab10410 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr 1/50 - 1/500.
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
IHC-FoFr 1/50 - 1/500.
ICC/IF 1/200.


  • 機能Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity.
  • 組織特異性Found in neuronal cell bodies and processes throughout the neocortex (at protein level). Expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. Weakly expressed in ovary. Down-regulated in brains from Parkinson disease and Alzheimer disease patients.
  • 関連疾患Parkinson disease 5
    Neurodegeneration with optic atrophy, childhood-onset
  • 配列類似性Belongs to the peptidase C12 family.
  • 翻訳後修飾O-glycosylated.
  • 細胞内局在Cytoplasm. Endoplasmic reticulum membrane. About 30% of total UCHL1 is associated with membranes in brain.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Epididymis luminal protein 117 antibody
    • Epididymis secretory protein Li 53 antibody
    • HEL 117 antibody
    • HEL S 53 antibody
    • NDGOA antibody
    • Neuron cytoplasmic protein 9.5 antibody
    • OTTHUMP00000218137 antibody
    • OTTHUMP00000218139 antibody
    • OTTHUMP00000218140 antibody
    • OTTHUMP00000218141 antibody
    • Park 5 antibody
    • PARK5 antibody
    • PGP 9.5 antibody
    • PGP9.5 antibody
    • PGP95 antibody
    • Protein gene product 9.5 antibody
    • Ubiquitin C terminal esterase L1 antibody
    • Ubiquitin C terminal hydrolase antibody
    • Ubiquitin C terminal hydrolase L1 antibody
    • Ubiquitin carboxyl terminal esterase L1 antibody
    • Ubiquitin carboxyl terminal hydrolase isozyme L1 antibody
    • Ubiquitin carboxyl-terminal hydrolase isozyme L1 antibody
    • Ubiquitin thioesterase L1 antibody
    • Ubiquitin thiolesterase antibody
    • Ubiquitin thiolesterase L1 antibody
    • UCH-L1 antibody
    • UCHL1 antibody
    • UCHL1_HUMAN antibody
    see all

Anti-PGP9.5 antibody - Neuronal Marker 画像

  • ab10410, at a dilution of 1/500, staining PGP9.5 in rat submucous plexus by Immunohistochemistry (PFA perfusion fixed frozen sections).
  • ICC/IF image of ab10410 stained DU145 cells. The cells were 4% paraformaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab10410, 1/200 dilution) overnight at +4°C. The secondary antibody (green) was ab96959, DyLight® 488 goat anti-guinea pig IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-PGP9.5 antibody - Neuronal Marker (ab10410) 使用論文

This product has been referenced in:
  • Buckinx R  et al. Transient receptor potential vanilloid type 1 channel (TRPV1) immunolocalization in the murine enteric nervous system is affected by the targeted C-terminal epitope of the applied antibody. J Histochem Cytochem 61:421-32 (2013). Read more (PubMed: 23482327) »

See 1 Publication for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: RT°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate buffer pH6.0
Sample Rat Tissue sections (Dorsal root ganglion)
Specification Dorsal root ganglion
Permeabilization Yes - 0,1% Tween -20
Fixative Paraformaldehyde

Yulia Sidorova

Verified customer

投稿 Feb 03 2014

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. I am sorry this product did not perform as stated on the datasheet and for the inconvenience this h...

Read More

As I mentioned in our phone call, your free of charge replacement for ab10410 should have been delivered today, 31st Oct 2011. If there is anything else I can help you with, please let me know.