Anti-PGP9.5 抗体 [BH7] (ab72911)
Key features and details
- Mouse monoclonal [BH7] to PGP9.5
- Suitable for: WB, ICC/IF, Flow Cyt
- Reacts with: Rat, Human
- Isotype: IgG1
製品の概要
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製品名
Anti-PGP9.5 antibody [BH7]
PGP9.5 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [BH7] to PGP9.5 -
由来種
Mouse -
アプリケーション
適用あり: WB, ICC/IF, Flow Cytmore details -
種交差性
交差種: Rat, Human -
免疫原
Recombinant full length human PGP9.5 purified from E. coli.
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ポジティブ・コントロール
- Rat hippocampal cell homogenate and rat spinal cord tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
Preservative: 0.065% Sodium azide
Constituent: PBS -
Concentration information loading...
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精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
BH7 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab72911の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/5000. Detects a band of approximately 43 kDa (predicted molecular weight: 25 kDa).
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ICC/IF |
1/500.
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Flow Cyt |
Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
特記事項 |
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WB
1/5000. Detects a band of approximately 43 kDa (predicted molecular weight: 25 kDa). |
ICC/IF
1/500. |
Flow Cyt
Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ターゲット情報
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機能
Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity. -
組織特異性
Found in neuronal cell bodies and processes throughout the neocortex (at protein level). Expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. Weakly expressed in ovary. Down-regulated in brains from Parkinson disease and Alzheimer disease patients. -
関連疾患
Parkinson disease 5
Neurodegeneration with optic atrophy, childhood-onset -
配列類似性
Belongs to the peptidase C12 family. -
翻訳後修飾
O-glycosylated. -
細胞内局在
Cytoplasm. Endoplasmic reticulum membrane. About 30% of total UCHL1 is associated with membranes in brain. - Information by UniProt
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参照データベース
- Entrez Gene: 7345 Human
- Entrez Gene: 29545 Rat
- Omim: 191342 Human
- SwissProt: P09936 Human
- SwissProt: Q00981 Rat
- Unigene: 518731 Human
- Unigene: 107213 Rat
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別名
- Epididymis luminal protein 117 antibody
- Epididymis secretory protein Li 53 antibody
- HEL 117 antibody
see all
画像
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Overlay histogram showing SH-SY5Y cells stained with ab72911 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab72911, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H&L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
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Anti-PGP9.5 antibody [BH7] (ab72911) at 1/5000 dilution + rat hippocampal homogenate
Predicted band size: 25 kDa
Observed band size: <43 kDa why is the actual band size different from the predicted? -
ab72911 at 1/500 dilution staining PGP9.5 in rat spinal cord by Immunofluorescence (shown in red). Anti-neurofilament NF-H antibody staining is shown in green. The large cells are a-motorneurons and PGP9.5 fills the cytoplasm of their perikarya and dendrites.
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (19)
ab72911 は 19 報の論文で使用されています。
- Lu Z et al. IL-20 promotes cutaneous inflammation and peripheral itch sensation in atopic dermatitis. FASEB J 36:e22334 (2022). PubMed: 35486004
- Chang WY et al. 5-HT7 receptor-dependent intestinal neurite outgrowth contributes to visceral hypersensitivity in irritable bowel syndrome. Lab Invest 102:1023-1037 (2022). PubMed: 35585132
- Rytel L et al. Endocrine Disruptor Bisphenol a Affects the Neurochemical Profile of Nerve Fibers in the Aortic Arch Wall in the Domestic Pig. Int J Environ Res Public Health 19:N/A (2022). PubMed: 35627499
- Alhilou AM et al. Density of nerve fibres and expression of substance P, NR2B-receptors and nerve growth factor in healthy human masseter muscle: An immunohistochemical study. J Oral Rehabil 48:35-44 (2021). PubMed: 33031568
- Donadio V et al. In Vivo Diagnosis of Synucleinopathies: A Comparative Study of Skin Biopsy and RT-QuIC. Neurology 96:e2513-e2524 (2021). PubMed: 33837116