製品の概要

  • 製品名
  • 製品の詳細
    Chicken polyclonal to PGP9.5
  • アプリケーション
    適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat, Cow, Human
  • 免疫原

    Recombinant full length human PGP9.5 purified from E. coli.

  • ポジティブ・コントロール
    • Rat hippocampal homogenate and Rat mixed neuron/glial cultures.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab72910 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2500. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).
ICC/IF 1/500.

ターゲット情報

  • 機能
    Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity.
  • 組織特異性
    Found in neuronal cell bodies and processes throughout the neocortex (at protein level). Expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. Weakly expressed in ovary. Down-regulated in brains from Parkinson disease and Alzheimer disease patients.
  • 関連疾患
    Parkinson disease 5
    Neurodegeneration with optic atrophy, childhood-onset
  • 配列類似性
    Belongs to the peptidase C12 family.
  • 翻訳後修飾
    O-glycosylated.
  • 細胞内局在
    Cytoplasm. Endoplasmic reticulum membrane. About 30% of total UCHL1 is associated with membranes in brain.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Epididymis luminal protein 117 antibody
    • Epididymis secretory protein Li 53 antibody
    • HEL 117 antibody
    • HEL S 53 antibody
    • NDGOA antibody
    • Neuron cytoplasmic protein 9.5 antibody
    • OTTHUMP00000218137 antibody
    • OTTHUMP00000218139 antibody
    • OTTHUMP00000218140 antibody
    • OTTHUMP00000218141 antibody
    • Park 5 antibody
    • PARK5 antibody
    • PGP 9.5 antibody
    • PGP9.5 antibody
    • PGP95 antibody
    • Protein gene product 9.5 antibody
    • Ubiquitin C terminal esterase L1 antibody
    • Ubiquitin C terminal hydrolase antibody
    • Ubiquitin C terminal hydrolase L1 antibody
    • Ubiquitin carboxyl terminal esterase L1 antibody
    • Ubiquitin carboxyl terminal hydrolase isozyme L1 antibody
    • Ubiquitin carboxyl-terminal hydrolase isozyme L1 antibody
    • Ubiquitin thioesterase L1 antibody
    • Ubiquitin thiolesterase antibody
    • Ubiquitin thiolesterase L1 antibody
    • UCH-L1 antibody
    • UCHL1 antibody
    • UCHL1_HUMAN antibody
    see all

Anti-PGP9.5 antibody 画像

  • Anti-PGP9.5 antibody (ab72910) at 1/2500 dilution + rat hippocampal homogenate

    Predicted band size : 25 kDa
    Observed band size : 25 kDa
  • ab72910 at 1/500 dilution staining PGP9.5 in rat mixed neuron/glial cultures by Immunofluorescence (green). Rabbit anti-GFAP antibody (red). Blue is a DNA stain.

Anti-PGP9.5 antibody (ab72910) 使用論文

This product has been referenced in:
  • Fu YY  et al. 3-D imaging and illustration of mouse intestinal neurovascular complex. Am J Physiol Gastrointest Liver Physiol 304:G1-11 (2013). ICC/IF ; Mouse . Read more (PubMed: 23086917) »
  • Allen C  et al. Neutrophil cerebrovascular transmigration triggers rapid neurotoxicity through release of proteases associated with decondensed DNA. J Immunol 189:381-92 (2012). Read more (PubMed: 22661091) »

See all 2 Publications for this product

Product Wall

Thank you for your phone call. For the neuronal cell body marker, I would recommend one of our chicken polyclonal to PGP9.5 antibodies: ab63497 or ab72910.

DISCOUNT CODE: ***
Expiration date: October 8th, 2012

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Read More
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (human skin)
Specification
human skin
Fixative
Paraformaldehyde
Permeabilization
Yes - triton 0.3 %
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
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投稿 Aug 25 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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