製品の概要

  • 製品名Anti-PGP9.5 antibody
    PGP9.5 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to PGP9.5
  • アプリケーション適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Rat, Cow, Human
  • 免疫原

    Recombinant full length protein (Human) purified from E. coli.

  • ポジティブ・コントロール
    • Whole bovine brain extract or rat mixed neuron/glial cultures.
  • 特記事項The total protein content in serum: 60mg/ml.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab64720 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
ICC/IF
  • 追加情報ICC/IF: 1/500.
    WB: 1/2000. Detects a band of approximately 24 kDa (predicted molecular weight: 25 kDa). The UCHL1 protein runs at about 24kDa on SDS-PAGE gels, and is a prominent component of brain, spinal cord and especially cortical extracts.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity.
    • 組織特異性Found in neuronal cell bodies and processes throughout the neocortex (at protein level). Expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. Weakly expressed in ovary. Down-regulated in brains from Parkinson disease and Alzheimer disease patients.
    • 関連疾患Parkinson disease 5
      Neurodegeneration with optic atrophy, childhood-onset
    • 配列類似性Belongs to the peptidase C12 family.
    • 翻訳後修飾O-glycosylated.
    • 細胞内局在Cytoplasm. Endoplasmic reticulum membrane. About 30% of total UCHL1 is associated with membranes in brain.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Epididymis luminal protein 117 antibody
      • Epididymis secretory protein Li 53 antibody
      • HEL 117 antibody
      • HEL S 53 antibody
      • NDGOA antibody
      • Neuron cytoplasmic protein 9.5 antibody
      • OTTHUMP00000218137 antibody
      • OTTHUMP00000218139 antibody
      • OTTHUMP00000218140 antibody
      • OTTHUMP00000218141 antibody
      • Park 5 antibody
      • PARK5 antibody
      • PGP 9.5 antibody
      • PGP9.5 antibody
      • PGP95 antibody
      • Protein gene product 9.5 antibody
      • Ubiquitin C terminal esterase L1 antibody
      • Ubiquitin C terminal hydrolase antibody
      • Ubiquitin C terminal hydrolase L1 antibody
      • Ubiquitin carboxyl terminal esterase L1 antibody
      • Ubiquitin carboxyl terminal hydrolase isozyme L1 antibody
      • Ubiquitin carboxyl-terminal hydrolase isozyme L1 antibody
      • Ubiquitin thioesterase L1 antibody
      • Ubiquitin thiolesterase antibody
      • Ubiquitin thiolesterase L1 antibody
      • UCH-L1 antibody
      • UCHL1 antibody
      • UCHL1_HUMAN antibody
      see all

    Anti-PGP9.5 antibody 画像

    • Anti-PGP9.5 antibody (ab64720) at 1/2000 dilution + Whole bovine brain extract

      Predicted band size : 25 kDa
      Observed band size : 24 kDa (why is the actual band size different from the predicted?)
    • Immunofluorescent staining of rat mixed neuron/glial cultures stained with ab64720 (1:500, green). Blue is a DNA stain. Note that the UCHL1 stains neurons strongly and specifically, and that the staining is concentrated in the cell bodies, though some does extend into the dendrites also. Surrounding glial cells are not stained with this antibody, though many are present as visualized using the DNA stain.

    Anti-PGP9.5 antibody (ab64720) 使用論文

    ab64720 has not yet been referenced specifically in any publications.

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    There are currently no Abreviews or Questions for ab64720.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"