Anti-PGP9.5 抗体 [346CT2.5.1] (ab86808)
Key features and details
- Mouse monoclonal [346CT2.5.1] to PGP9.5
- Suitable for: ICC/IF, Flow Cyt (Intra), WB
- Knockout validated
- Reacts with: Rat, Human
- Isotype: IgG1
Related conjugates and formulations
製品の概要
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製品名
Anti-PGP9.5 antibody [346CT2.5.1]
PGP9.5 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [346CT2.5.1] to PGP9.5 -
由来種
Mouse -
アプリケーション
適用あり: ICC/IF, Flow Cyt (Intra), WBmore details -
種交差性
交差種: Rat, Human
交差が予測される動物種: Mouse, Goat, Horse, Cat, Dog, Pig -
免疫原
Recombinant full length protein corresponding to Human PGP9.5 aa 1 to the C-terminus.
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ポジティブ・コントロール
- This antibody gave a positive signal in the following lysates: Human Brain Tissue; Rat Brain Tissue; Rat Brain Cortex Tissue; PC12 Whole Cell; Y79 Whole Cell; U-87MG Whole Cell; SHSY-5Y Whole Cell and HAP1 Wole cells.
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特記事項
This monoclonal antibody to PGP9.5 has been knockout validated in Western blot. The expected band for PGP9.5 was observed in wild type cells and the band was not seen in knockout cells.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
346CT2.5.1 -
ミエローマ
Sp2/0 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab86808の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF | (1) |
Use at an assay dependent concentration.
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Flow Cyt (Intra) |
Use 0.01-0.1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).
Abcam recommends blocking with 3% Milk. |
特記事項 |
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ICC/IF
Use at an assay dependent concentration. |
Flow Cyt (Intra)
Use 0.01-0.1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa). Abcam recommends blocking with 3% Milk. |
ターゲット情報
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機能
Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity. -
組織特異性
Found in neuronal cell bodies and processes throughout the neocortex (at protein level). Expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. Weakly expressed in ovary. Down-regulated in brains from Parkinson disease and Alzheimer disease patients. -
関連疾患
Parkinson disease 5
Neurodegeneration with optic atrophy, childhood-onset -
配列類似性
Belongs to the peptidase C12 family. -
翻訳後修飾
O-glycosylated. -
細胞内局在
Cytoplasm. Endoplasmic reticulum membrane. About 30% of total UCHL1 is associated with membranes in brain. - Information by UniProt
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参照データベース
- Entrez Gene: 100033838 Horse
- Entrez Gene: 7345 Human
- Entrez Gene: 22223 Mouse
- Entrez Gene: 396637 Pig
- Entrez Gene: 29545 Rat
- Omim: 191342 Human
- SwissProt: Q9GM50 Horse
- SwissProt: P09936 Human
see all -
別名
- Epididymis luminal protein 117 antibody
- Epididymis secretory protein Li 53 antibody
- HEL 117 antibody
see all
画像
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Lane 1: Wild type HAP1 whole cell lysate (20 µg)
Lane 2: UCHL1 (KO) knockout HAP1 whole cell lysate (20 µg)
Lane 3: SH-SY5Y whole cell lysate (20 µg)
Lanes 1 - 3: Merged signal (red and green). Green - ab86808 observed at 24 kDa. Red - loading control, ab181602, observed at 37 kDa.Ab86808 was shown to specifically react with PGP9.5/UCHL1 in wild-type cells as signal was lost in PGP9/UCHL1 knockout HAP1 cells. Wild-type and UCHL1 (KO) knockout samples were subjected to SDS-PAGE. Ab86808 and ab181602 (Rabbit anti GAPDH loading control) were incubated overnight at 4°C at 1 ug/ml and 1/2000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed ab216772 and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed ab216777 secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
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Overlay histogram showing SH-SH5Y cells stained with ab86808 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab86808, 0.1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in SH-SY5Y cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
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All lanes : Anti-PGP9.5 antibody [346CT2.5.1] (ab86808) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Brain (Rat) Tissue Lysate
Lane 3 : Rat Cortex Tissue Lysate
Lane 4 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
Lane 5 : Y79 (Human retinoblastoma cell line) Whole Cell Lysate
Lane 6 : U-87 MG (Human glioblastoma astrocytoma) Whole Cell Lysate
Lane 7 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 25 kDa
Observed band size: 25 kDa
Exposure time: 1 minute
データシートおよび資料
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Datasheet download
参考文献 (3)
ab86808 は 3 報の論文で使用されています。
- Bautzova T et al. 5-oxoETE triggers nociception in constipation-predominant irritable bowel syndrome through MAS-related G protein-coupled receptor D. Sci Signal 11:N/A (2018). PubMed: 30563864
- Desormeaux C et al. Protease-activated receptor 1 is implicated in irritable bowel syndrome mediators-induced signaling to thoracic human sensory neurons. Pain 159:1257-1267 (2018). PubMed: 29554016
- Cenac N et al. Quantification and Potential Functions of Endogenous Agonists of Transient Receptor Potential Channels in Patients With Irritable Bowel Syndrome. Gastroenterology 149:433-44.e7 (2015). PubMed: 25911511