製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to PGK1
  • アプリケーション
    適用あり: ICC/IF, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Sheep, Horse, Cow, Pig, Non human primates
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human PGK1.

    (Peptide available as ab100815.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following whole cell lysates: HepG2; Caco 2; HeLa; Jurkat; HEK293.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab90787 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 46 kDa (predicted molecular weight: 44 kDa).

ターゲット情報

  • 機能
    In addition to its role as a glycolytic enzyme, it seems that PGK-1 acts as a polymerase alpha cofactor protein (primer recognition protein).
  • パスウェイ
    Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 2/5.
  • 関連疾患
    Defects in PGK1 are the cause of phosphoglycerate kinase 1 deficiency (PGK1D) [MIM:300653]. It is a condition with a highly variable clinical phenotype that includes hemolytic anemia, rhabdomyolysis, myopathy and neurologic involvement. Patients can express one or more of these manifestations.
  • 配列類似性
    Belongs to the phosphoglycerate kinase family.
  • 細胞内局在
    Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Cell migration-inducing gene 10 protein antibody
    • Epididymis secretory sperm binding protein Li 68p antibody
    • HEL S 68p antibody
    • MGC117307 antibody
    • MGC8947 antibody
    • MIG10 antibody
    • pgk1 antibody
    • PGK1_HUMAN antibody
    • PGKA antibody
    • Phosphoglycerate kinase 1 antibody
    • Primer recognition protein 2 antibody
    • PRP 2 antibody
    see all

画像

  • All lanes : Anti-PGK1 antibody (ab90787) at 1 µg/ml

    Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 2 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
    Lane 3 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 5 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 44 kDa
    Observed band size : 46 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 42 kDa,52 kDa,75 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 2 minutes
  • ICC/IF image of ab90787 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab90787 at 5µg/ml overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti- rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in formaldehyde fixed (4%) (10 min) HeLa cells.

  • Anti-PGK1 antibody (ab90787) at 1 µg/ml + Recombinant Human PGK1 protein (ab87630) at 0.1 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 30 seconds

参考文献

This product has been referenced in:
  • Hinckelmann MV  et al. Self-propelling vesicles define glycolysis as the minimal energy machinery for neuronal transport. Nat Commun 7:13233 (2016). WB ; Mouse . Read more (PubMed: 27775035) »

See 1 Publication for this product

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