製品の概要

  • 製品名Anti-PGK1 antibody [22C5D8]
    PGK1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [22C5D8] to PGK1
  • アプリケーション適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Saccharomyces cerevisiae
  • 免疫原

    Full length native protein (purified)

  • 特記事項

    Product was previously marketed under the MitoSciences sub-brand.

     

    Alternative versions available:

    Anti-PGK1 antibody (HRP) [22C5D8] (ab197960)

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab113687 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).
ICC/IF Use a concentration of 5 - 10 µg/ml.

ターゲット情報

  • 機能In addition to its role as a glycolytic enzyme, it seems that PGK-1 acts as a polymerase alpha cofactor protein (primer recognition protein).
  • パスウェイCarbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 2/5.
  • 関連疾患Defects in PGK1 are the cause of phosphoglycerate kinase 1 deficiency (PGK1D) [MIM:300653]. It is a condition with a highly variable clinical phenotype that includes hemolytic anemia, rhabdomyolysis, myopathy and neurologic involvement. Patients can express one or more of these manifestations.
  • 配列類似性Belongs to the phosphoglycerate kinase family.
  • 細胞内局在Cytoplasm.
  • Information by UniProt
  • 参照データベース
    • 別名
      • Cell migration-inducing gene 10 protein antibody
      • Epididymis secretory sperm binding protein Li 68p antibody
      • HEL S 68p antibody
      • MGC117307 antibody
      • MGC8947 antibody
      • MIG10 antibody
      • pgk1 antibody
      • PGK1_HUMAN antibody
      • PGKA antibody
      • Phosphoglycerate kinase 1 antibody
      • Primer recognition protein 2 antibody
      • PRP 2 antibody
      see all

    Anti-PGK1 antibody [22C5D8] 画像

    • Anti-PGK1 antibody [22C5D8] (ab113687) + Yeast whole cell lysate at 15 µg
      Developed using the ECL technique

      Performed under reducing conditions.

      Predicted band size : 45 kDa

    Anti-PGK1 antibody [22C5D8] (ab113687) 使用論文

    This product has been referenced in:
    • Belgareh-Touzé N  et al. Ubiquitination of ERMES components by the E3 ligase Rsp5 is involved in mitophagy. Autophagy 13:114-132 (2017). WB ; Saccharomyces cerevisiae . Read more (PubMed: 27846375) »
    • Sen A & Cox RT Clueless is a conserved ribonucleoprotein that binds the ribosome at the mitochondrial outer membrane. Biol Open 5:195-203 (2016). Read more (PubMed: 26834020) »

    See all 14 Publications for this product

    Product Wall

    Application Western blot
    Sample Saccharomyces cerevisiae Cell lysate - nuclear (Saccharomyces cerevisiae)
    Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris)
    Loading amount 30 µg
    Specification Saccharomyces cerevisiae
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    投稿 Jun 09 2017

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"