Anti-PGC1 alpha + beta 抗体 (ab72230)
Key features and details
- Rabbit polyclonal to PGC1 alpha + beta
- Suitable for: ELISA, WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-PGC1 alpha + beta antibody
PGC1 alpha + beta 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PGC1 alpha + beta -
由来種
Rabbit -
特異性
The immunogen design for ab72230 is shown to cross-react with both PGC1 alpha (ID: 124782 and Swissprot Q9UBK2) and PGC1 beta (ID: 240776 and SwissProt Q86YN6). From Jan 2024, QC testing of replenishment batches of this polyclonal changed. All tested and expected application and reactive species combinations are still covered by our Abcam product promise. However, we no longer test all applications. For more information on a specific batch, please contact our Scientific Support who will be happy to help. You may also be interested in our alternative recombinant antibody, ab188102.
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アプリケーション
適用あり: ELISA, WB, ICC/IFmore details -
種交差性
交差種: Mouse, Rat, Human
交差が予測される動物種: Chicken, Cow, Dog, Pig, Rhesus monkey -
免疫原
Synthetic peptide conjugated to KLH derived from within residues 750 to the C-terminus of Human PGC1 alpha.
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ポジティブ・コントロール
- This antibody gave a positive signal in the following Tissue Lysates: Human Skeletal Muscle Mouse Testis Mouse Colon Mouse Skeletal Muscle Rat Ovary Rat Thymus
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特記事項
PGC1 alpha has a predicted MW of 92 kDa and PGC1 beta has a predicted MW of 113 kDa.
This product may be covered by one or more of the following U.S. Patents.
6,166,192
6,426,411
6,525,178
6,872,391
6,908,987
7,250,283
7,091,006
7,354,738
8,105,774
8,445,233The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
- Epigenetics and Nuclear Signaling
- Nuclear Signaling Pathways
- Nuclear Receptors
- Co-activators/co-repressors
- Metabolism
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Fatty acids
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab72230の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ELISA |
Use a concentration of 1 µg/ml.
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WB | (2) |
Use a concentration of 1 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 91 kDa).
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ICC/IF |
1/100.
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特記事項 |
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ELISA
Use a concentration of 1 µg/ml. |
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 91 kDa). |
ICC/IF
1/100. |
ターゲット情報
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関連性
PGC1 alpha - The protein encoded by this gene is a transcriptional coactivator that regulates the genes involved in energy metabolism. This protein interacts with PPARgamma, which permits the interaction of this protein with multiple transcription factors. This protein can interact with, and regulate the activities of cAMP response element binding protein (CREB) and nuclear respiratory factors (NRFs). It provides a direct link between external physiological stimuli and the regulation of mitochondrial biogenesis, and is a major factor that regulates muscle fiber type determination. This protein may be also involved in controlling blood pressure, regulating cellular cholesterol homoeostasis, and the development of obesity (referenced from entrez gene). PGC1 beta - Plays a role of stimulator of transcription factors and nuclear receptors activities. Activates transcritional activity of estrogen receptor alpha, nuclear respiratory factor 1 (NRF1) and glucocorticoid receptor in the presence of glucocorticoids. May play a role in constitutive non-adrenergic-mediated mitochondrial biogenesis as suggested by increased basal oxygen consumption and mitochondrial number when overexpressed. May be involved in fat oxidation and non-oxidative glucose metabolism and in the regulation of energy expenditure. -
細胞内局在
Nucleus -
参照データベース
- Entrez Gene: 10891 Human
- Entrez Gene: 133522 Human
- Entrez Gene: 170826 Mouse
- Entrez Gene: 19017 Mouse
- Entrez Gene: 291567 Rat
- Entrez Gene: 83516 Rat
- Omim: 604517 Human
- Omim: 608886 Human
see all -
別名
- LEM6 antibody
- Ligand effect modulator 6 antibody
- PERC antibody
see all
画像
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Immunofluorescence analysis of 4% paraformaldehyde fixed HeLa cells (Human epithelial cells from cervix adenocarcinoma) labeling PGC1 alpha+beta with ab72230 at a 1/100 dilution showing nuclear staining. Permeabilisation using 0.1% tritonX-100.
Secondary ab: Anti-Rabbit Alexa Fluor® 488 (ab150077) at 1/500 dilution. Counter stain is labeling tubulin (red) with ab7291 at 1/1000 dilution with secondary antibody Anti-Mouse AlexaFluor® 594 (ab150120) at 1/500 dilution. DAPI stains the nucleus in blue. -ve control 1 is ab72230 at 1/100 dilution, Anti-Mouse AlexaFluor® 594 (ab150120) at 1/500 dilution. -ve control 2 is ab7291 at 1/1000 dilution, Anti-Rabbit Alexa Fluor® 488 (ab150077) at 1/500 dilution.
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All lanes : Anti-PGC1 alpha + beta antibody (ab72230) at 1 µg/ml
Lane 1 : Testis (Mouse) Tissue Lysate
Lane 2 : Human skeletal muscle tissue lysate - total protein (ab29330)
Lane 3 : Ovary (Rat) Tissue Lysate
Lane 4 : Colon (Mouse) Tissue Lysate
Lane 5 : Skeletal Muscle (Mouse) Tissue Lysate
Lane 6 : Thymus (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 91 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?
Additional bands at: 35 kDa, 45 kDa, 55 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 2 minutes
We have occasionally observed bands in the region of 90 kDa in batches of this antibody, which corresponds to the molecular weight based on primary sequence. -
All lanes : Anti-PGC1 alpha + beta antibody (ab72230) at 1 µg/ml
Lane 1 : Human skeletal muscle tissue lysate - total protein (ab29330)
Lane 2 : Colon (Mouse) Tissue Lysate
Lane 3 : Thymus (Rat) Tissue Lysate
Lane 4 : Human skeletal muscle tissue lysate - total protein (ab29330) with Human PGC1 alpha peptide (ab73600) at 1 µg/ml
Lane 5 : Colon (Mouse) Tissue Lysate with Human PGC1 alpha peptide (ab73600) at 1 µg/ml
Lane 6 : Thymus (Rat) Tissue Lysate with Human PGC1 alpha peptide (ab73600) at 1 µg/ml
Lane 7 : Human skeletal muscle tissue lysate - total protein (ab29330) with PGC1 beta peptide at 1 µg/ml
Lane 8 : Colon (Mouse) Tissue Lysate with PGC1 beta peptide at 1 µg/ml
Lane 9 : Thymus (Rat) Tissue Lysate with PGC1 beta peptide at 1 µg/ml
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 91 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?
Additional bands at: 45 kDa, 73 kDa, 90 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes
Western Blot showing equal blocking with the PGC1 alpha and PGC1 beta peptides. -
ab72230 was tested using an Indirect ELISA approach. The wells were coated with peptide (1µg/ml at 100µl/well) overnight at 4°C, followed by a 5% BSA blocking step for 1 hour at room temperature. The primary Ab was then added at a dilution range of 1- 0.00025µg/ml (100µl/well) for 1hr at room temperature. A HRP-conjugated anti-rabbit IgG (heavy and light chain) was used as a secondary antibody at 1:20,000 dilution for 1hr at room temperature.
Ab72230 gave a positive result in ELISA against PGC1 alpha peptide and PGC1 beta peptide. This indicates that it recognises both forms.
Orange line: ELISA against immunising peptide ab73600
Yellow line: ELISA against ab88232
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (38)
ab72230 は 38 報の論文で使用されています。
- Cheng L et al. Rutin-activated adipose tissue thermogenesis is correlated with increased intestinal short-chain fatty acid levels. Phytother Res 36:2495-2510 (2022). PubMed: 35445769
- Joseph LC et al. Combined metabolomic and transcriptomic profiling approaches reveal the cardiac response to high-fat diet. iScience 25:104184 (2022). PubMed: 35494220
- Qi G et al. ApoE4 Impairs Neuron-Astrocyte Coupling of Fatty Acid Metabolism. Cell Rep 34:108572 (2021). PubMed: 33406436
- Lee DI et al. Cannabinoid Receptor Agonist Inhibits Atrial Electrical Remodeling in a Tachypaced Ex Vivo Rat Model. Front Pharmacol 12:642398 (2021). PubMed: 33967775
- Jayakumari NR et al. Honokiol regulates mitochondrial substrate utilization and cellular fatty acid metabolism in diabetic mice heart. Eur J Pharmacol 896:173918 (2021). PubMed: 33529726