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This product may be covered by one or more of the following U.S. Patents.
Our Abpromise guarantee covers the use of ab54481 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 92 kDa.
Use of nuclear extracts for WB is recommended since protein is nuclear and expression within whole cell lysate can be low.
|ICC/IF||1/300. PubMed: 22545021|
|IHC-P||Use at an assay dependent concentration.|
Western blot using ab54481 at 1:2000 to detect PGC1 alpha in Mouse brown adipose tissue whole cell extract (both lanes).
Immunofluorescence analysis of Human white adipose tissue progenitor cells, staining PGC1 alpha (green) with ab54481.
Cells were fixed in paraformaldehyde and permeabilized in 0.1% Triton X-100 for 5 min. Cells were incubated with primary antibody (1/300) and staining was detected with Alexa Fluor® 546-conjugated anti-rabbit secondary antibody (1/1000).