製品の概要

  • 製品名Anti-PGC1 alpha antibody
    PGC1 alpha 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to PGC1 alpha
  • 特異性The immunogen sequence for this antibody is 78% homologous with PGC1 beta (Uniprot: Q86YN6).
  • アプリケーション適用あり: WB, ELISA, ICC/IF, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat, Human, Zebrafish
    交差が予測される動物種: Horse, Chicken, Cow, Dog, Pig, Cynomolgus Monkey, Rhesus monkey, Opossum
  • 免疫原

    Synthetic peptide corresponding to Human PGC1 alpha aa 777-797.
    Sequence:

    C-SKYDSLDFDSLLKEAQRSLRR


    Database link: Q9UBK2

  • 特記事項

    This product may be covered by one or more of the following U.S. Patents.

    6,166,192
    6,426,411
    6,525,178
    6,872,391
    6,908,987
    7,250,283
    7,091,006
    7,354,738
    8,105,774
    8,445,233

     

アプリケーション

Our Abpromise guarantee covers the use of ab54481 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Predicted molecular weight: 92 kDa.

Use of nuclear extracts for WB is recommended since protein is nuclear and expression within whole cell lysate can be low.

ELISA 1/50000.
ICC/IF 1/300. PubMed: 22545021
IHC-P Use at an assay dependent concentration.

ターゲット情報

  • 機能Transcriptional coactivator for steroid receptors and nuclear receptors. Greatly increases the transcriptional activity of PPARG and thyroid hormone receptor on the uncoupling protein promoter. Can regulate key mitochondrial genes that contribute to the program of adaptive thermogenesis. Plays an essential role in metabolic reprogramming in response to dietary availability through coordination of the expression of a wide array of genes involved in glucose and fatty acid metabolism. Induces the expression of PERM1 in the skeletal muscle in an ESRRA-dependent manner. Also involved in the integration of the circadian rhythms and energy metabolism. Required for oscillatory expression of clock genes, such as ARNTL/BMAL1 and NR1D1, through the coactivation of RORA and RORC, and metabolic genes, such as PDK4 and PEPCK.
  • 組織特異性Heart, skeletal muscle, liver and kidney. Expressed at lower levels in brain and pancreas and at very low levels in the intestine and white adipose tissue. In skeletal muscle, levels were lower in obese than in lean subjects and fasting induced a 2-fold increase in levels in the skeletal muscle in obese subjects.
  • 配列類似性Contains 1 RRM (RNA recognition motif) domain.
  • 翻訳後修飾Phosphorylation by AMPK in skeletal muscle increases activation of its own promoter. Phosphorylated by CLK2.
    Heavily acetylated by GCN5 and biologically inactive under conditions of high nutrients. Deacetylated by SIRT1 in low nutrients/high NAD conditions.
    Ubiquitinated. Ubiquitination by RNF34 induces proteasomal degradation.
  • 細胞内局在Cytoplasm. Nucleus; Nucleus and Nucleus. Nucleus, PML body.
  • Information by UniProt
  • 参照データベース
  • 別名
    • L PGC 1alpha antibody
    • LEM6 antibody
    • Ligand effect modulator 6 antibody
    • Peroxisome proliferative activated receptor gamma coactivator 1 alpha antibody
    • Peroxisome proliferative activated receptor gamma coactivator 1 antibody
    • Peroxisome proliferator activated receptor gamma coactivator 1 alpha antibody
    • Peroxisome proliferator activated receptor gamma coactivator 1 alpha transcript variant B4 3ext antibody
    • Peroxisome proliferator activated receptor gamma coactivator 1 alpha transcript variant B4 8a antibody
    • Peroxisome proliferator activated receptor gamma coactivator 1 alpha transcript variant B4 antibody
    • Peroxisome proliferator activated receptor gamma coactivator 1 alpha transcript variant B5 antibody
    • Peroxisome proliferator activated receptor gamma coactivator 1 alpha transcript variant B5 NT antibody
    • Peroxisome proliferator-activated receptor gamma coactivator 1-alpha antibody
    • PGC 1 (alpha) antibody
    • PGC 1 alpha antibody
    • PGC 1v antibody
    • PGC-1-alpha antibody
    • PGC1 antibody
    • PGC1(alpha) antibody
    • PGC1A antibody
    • PGC1v antibody
    • PPAR gamma coactivator 1 alpha 3 ligand effect modulator 6 antibody
    • PPAR gamma coactivator 1 alpha antibody
    • PPAR gamma coactivator 1 antibody
    • PPAR gamma coactivator variant form antibody
    • PPAR-gamma coactivator 1-alpha antibody
    • PPARGC 1 alpha antibody
    • PPARGC-1-alpha antibody
    • PPARGC1 antibody
    • PPARGC1A antibody
    • PRGC1_HUMAN antibody
    see all

Anti-PGC1 alpha antibody 画像

  • All lanes : Anti-PGC1 alpha antibody (ab54481) at 1/2000 dilution

    Lane 1 : Mouse Brown Adipose Tissue at 10 µg
    Lane 2 : Mouse Brown Adipose Tissue at 20 µg


    Predicted band size : 92 kDa
    Observed band size : 105 kDa (why is the actual band size different from the predicted?)

    Western blot using ab54481 at 1:2000 to detect PGC1 alpha in Mouse brown adipose tissue whole cell extract (both lanes).

  • Immunofluorescence analysis of Human white adipose tissue progenitor cells, staining PGC1 alpha (green) with ab54481.
    Cells were fixed in paraformaldehyde and permeabilized in 0.1% Triton X-100 for 5 min. Cells were incubated with primary antibody (1/300) and staining was detected with Alexa Fluor® 546-conjugated anti-rabbit secondary antibody (1/1000).

  • Immunohistochemical analysis of mouse kidney tissue, staining PGC1 alpha with ab54481.

    Tissue was fixed with 10% Neutral Buffered Formalin and blocked with 0.25% protein block for 20 minutes at 25°C; antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/250 in 1% BSA + 0.5% skim milk) for 16 hours at 4°C. An HRP-conjugated goat anti-rabbit polyclonal IgG was used as the secondary antibody.

    See Abreview

Anti-PGC1 alpha antibody (ab54481) 使用論文

This product has been referenced in:
  • Liu Z  et al. Mark4 promotes oxidative stress and inflammation via binding to PPAR? and activating NF-?B pathway in mice adipocytes. Sci Rep 6:21382 (2016). WB ; Mouse . Read more (PubMed: 26888669) »
  • Helman A  et al. p16(Ink4a)-induced senescence of pancreatic beta cells enhances insulin secretion. Nat Med N/A:N/A (2016). WB . Read more (PubMed: 26950362) »

See all 31 Publications for this product

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Application Western blot
Sample Mouse Tissue lysate - whole (anterior tibialis)
Gel Running Conditions Reduced Denaturing (precast)
Loading amount 50 µg
Specification anterior tibialis
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
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投稿 Nov 29 2016

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Rat Tissue sections (Brain section)
Antigen retrieval step None
Specification Brain section
Blocking step Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Fixative Paraformaldehyde
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投稿 Apr 08 2015

Application Western blot
Loading amount 25 µg
Gel Running Conditions Reduced Denaturing
Sample Mouse Cell lysate - whole cell (Cardiomyocytes)
Specification Cardiomyocytes
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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投稿 Mar 16 2015

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (8% Gel)
Sample Mouse Tissue lysate - whole (Skeletal muscle)
Specification Skeletal muscle
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
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投稿 Feb 05 2015

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (8% Gel)
Sample Rat Tissue lysate - whole (skeletal muscle)
Specification skeletal muscle
Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
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投稿 Feb 05 2015

Application Western blot
Loading amount 25 µg
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - nuclear (liver)
Specification liver
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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投稿 Dec 25 2014

Application Western blot
Loading amount 25 µg
Gel Running Conditions Reduced Denaturing
Sample Mouse Cell lysate - nuclear (hepatocytes)
Specification hepatocytes
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
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投稿 Dec 23 2014

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunoprecipitation
Sample Rat Tissue lysate - nuclear (Liver)
Total protein in input 500 µg
Immuno-precipitation step Protein A
Specification Liver
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投稿 Nov 11 2014

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (9% SDS-PAGE)
Sample Rat Tissue lysate - whole (Rat skeletal muscle)
Specification Rat skeletal muscle
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 Nov 10 2014

1-10 of 64 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"