製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to PGBD1
  • アプリケーション
    適用あり: WB, IPmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Horse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla
  • 免疫原

    Synthetic peptide, corresponding to a region within amino acids 275-325 of Human PGBD1 (NP_001171672.1).

  • ポジティブ・コントロール
    • HeLa, Jurkat or 293T whole cell lysate.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    pH: 7.50
    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab115173 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/5000. Predicted molecular weight: 93 kDa.
IP Use at 2-10 µg/mg of lysate.

ターゲット情報

画像

  • All lanes : Anti-PGBD1 antibody (ab115173) at 0.4 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : 293T whole cell lysate at 50 µg
    Lane 4 : Jurkat whole cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 93 kDa


    Exposure time : 3 minutes
  • Samples: whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded).
    Antibodies: ab115173 used for WB at 1 µg/ml and used for IP at 6 µg/mg lysate.
    Detection: chemiluminescence with exposure time of 30 seconds (B).

参考文献

ab115173 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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