Anti-PGAM2 抗体 (ab97800)
Key features and details
- Rabbit polyclonal to PGAM2
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-PGAM2 antibody
PGAM2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to PGAM2 -
由来種
Rabbit -
特異性
The immunogen sequence shows 81% homology with PGAM1, so cross-reactivity with PGAM1 cannot be excluded. However, cross-reactivity has not been tested. -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Cow, Pig -
免疫原
Recombinant fragment, corresponding to a region within amino acids 1-203 of Human PGAM2 (NP_000281)
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ポジティブ・コントロール
- H1299 or HeLa lysates A549 cells (IF)
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab97800の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/3000. Predicted molecular weight: 29 kDa.
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ICC/IF |
1/100 - 1/200.
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特記事項 |
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WB
1/500 - 1/3000. Predicted molecular weight: 29 kDa. |
ICC/IF
1/100 - 1/200. |
ターゲット情報
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機能
Interconversion of 3- and 2-phosphoglycerate with 2,3-bisphosphoglycerate as the primer of the reaction. Can also catalyze the reaction of EC 5.4.2.4 (synthase) and EC 3.1.3.13 (phosphatase), but with a reduced activity. -
組織特異性
In mammalian tissues there are two types of phosphoglycerate mutase isozymes: type-M in muscles and type-B in other tissues. -
関連疾患
Defects in PGAM2 are the cause of glycogen storage disease type 10 (GSD10) [MIM:261670]. A metabolic disorder characterized by myoglobinuria, increased serum creatine kinase levels, decreased phosphoglycerate mutase activity, myalgia, muscle pain, muscle cramps and excercise intolerance. -
配列類似性
Belongs to the phosphoglycerate mutase family. BPG-dependent PGAM subfamily. - Information by UniProt
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参照データベース
- Entrez Gene: 515067 Cow
- Entrez Gene: 5224 Human
- Entrez Gene: 56012 Mouse
- Entrez Gene: 100188980 Pig
- Entrez Gene: 24959 Rat
- Omim: 612931 Human
- SwissProt: Q32KV0 Cow
- SwissProt: P15259 Human
see all -
別名
- BPG dependent PGAM 2 antibody
- BPG-dependent PGAM 2 antibody
- GSD10 antibody
see all
画像
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All lanes : Anti-PGAM2 antibody (ab97800) at 1/10000 dilution
Lane 1 : H1299 whole cell lysate
Lane 2 : HeLa whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 29 kDa
12% SDS PAGE -
Immunofluorescence analysis of paraformaldehyde-fixed A549, using ab97800 at 1/200 dilution. Lower image was also merged with DNA probe.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (3)
ab97800 は 3 報の論文で使用されています。
- Rudolph F et al. Deconstructing sarcomeric structure-function relations in titin-BioID knock-in mice. Nat Commun 11:3133 (2020). PubMed: 32561764
- De Ridder W et al. Multisystem proteinopathy due to a homozygous p.Arg159His VCP mutation: A tale of the unexpected. Neurology 94:e785-e796 (2020). PubMed: 31848255
- Xu Y et al. Oxidative stress activates SIRT2 to deacetylate and stimulate phosphoglycerate mutase. Cancer Res 74:3630-42 (2014). PubMed: 24786789