製品の概要

  • 製品名
    Anti-PGAM1 antibody
    PGAM1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to PGAM1
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Zebrafish
  • 免疫原

    Recombinant fragment, corresponding to a region within amino acids 16-209 of Human PGAM1 (AAH00455).

  • ポジティブ･コントロール
    • 293T, A431, Jurkat and Raji cell lysates

法規制情報

医薬用外毒物

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab96622 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/3000. Predicted molecular weight: 29 kDa.

ターゲット情報

  • 機能
    Interconversion of 3- and 2-phosphoglycerate with 2,3-bisphosphoglycerate as the primer of the reaction. Can also catalyze the reaction of EC 5.4.2.4 (synthase) and EC 3.1.3.13 (phosphatase), but with a reduced activity.
  • 組織特異性
    In mammalian tissues there are two types of phosphoglycerate mutase isozymes: type-M in muscles and type-B in other tissues.
  • 配列類似性
    Belongs to the phosphoglycerate mutase family. BPG-dependent PGAM subfamily.
  • Information by UniProt
  • 参照データベース
    see all
  • 別名
    • BPG dependent PGAM 1 antibody
    • BPG dependent PGAM1 antibody
    • BPG-dependent PGAM 1 antibody
    • OTTHUMP00000059414 antibody
    • PGAM 1 antibody
    • PGAM A antibody
    • PGAM B antibody
    • PGAM-B antibody
    • PGAM1 antibody
    • PGAM1_HUMAN antibody
    • PGAMA antibody
    • PGAMB antibody
    • Phosphoglycerate mutase 1 antibody
    • Phosphoglycerate mutase 1 brain antibody
    • Phosphoglycerate mutase A antibody
    • Phosphoglycerate mutase A nonmuscle form antibody
    • Phosphoglycerate mutase isozyme B antibody
    see all

画像

  • Western blot - PGAM1 antibody (ab96622)
    Western blot - PGAM1 antibody (ab96622)
    All lanes : Anti-PGAM1 antibody (ab96622) at 1/1000 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : A431 whole cell lysate
    Lane 3 : Jurkat whole cell lysate
    Lane 4 : Raji whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 29 kDa

  • Western blot
    Western blot
    All lanes : Anti-PGAM1 antibody (ab96622) at 1/1000 dilution

    Lane 1 : Recombinant Human PGAM1 protein (ab100885) at 0.1 µg
    Lane 2 : Recombinant Human PGAM1 protein (ab100885) at 0.01 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 29 kDa


    Exposure time: 1 minute

プロトコール

参考文献

This product has been referenced in:
  • Jiang W  et al. Proteomic analysis of protein expression profiles during hyperthermia-induced apoptosis in Tca8113 cells. Oncol Lett 6:135-143 (2013). Read more (PubMed: 23946791) »

See 1 Publication for this product

ab96622 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

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Western blot abreview for Anti-PGAM1 antibody

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Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Jurkat Cells)
Loading amount
30 µg
Specification
Jurkat Cells
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 22°C
Read More
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投稿 Aug 10 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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