製品の概要
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製品名Anti-PGAM1 antibody
PGAM1 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to PGAM1
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アプリケーション適用あり: WBmore details
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種交差性交差種: Human
交差が予測される動物種: Mouse, Rat, Zebrafish
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免疫原
Recombinant fragment, corresponding to a region within amino acids 16-209 of Human PGAM1 (AAH00455).
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ポジティブ･コントロール
- 293T, A431, Jurkat and Raji cell lysates
法規制情報
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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バッファーPreservative: 0.01% Thimerosal (merthiolate)
Constituents: 20% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7 -
Concentration information loading... -
精製度Immunogen affinity purified
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特記事項（精製）Purified by antigen affinity chromatography.
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
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Positive Controls
アプリケーション
Our Abpromise guarantee covers the use of ab96622 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 29 kDa. |
ターゲット情報
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機能Interconversion of 3- and 2-phosphoglycerate with 2,3-bisphosphoglycerate as the primer of the reaction. Can also catalyze the reaction of EC 5.4.2.4 (synthase) and EC 3.1.3.13 (phosphatase), but with a reduced activity.
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組織特異性In mammalian tissues there are two types of phosphoglycerate mutase isozymes: type-M in muscles and type-B in other tissues.
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配列類似性Belongs to the phosphoglycerate mutase family. BPG-dependent PGAM subfamily.
- Information by UniProt
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参照データベース
- Entrez Gene: 5223 Human
- Entrez Gene: 18648 Mouse
- Entrez Gene: 24642 Rat
- Omim: 172250 Human
- SwissProt: P18669 Human
- SwissProt: Q9DBJ1 Mouse
- SwissProt: P25113 Rat
- Unigene: 592599 Human
see all -
別名
- BPG dependent PGAM 1 antibody
- BPG dependent PGAM1 antibody
- BPG-dependent PGAM 1 antibody
see all
Anti-PGAM1 antibody 画像
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Western blot - PGAM1 antibody (ab96622)All lanes : Anti-PGAM1 antibody (ab96622) at 1/1000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : Raji whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size : 29 kDa -
Western blotAll lanes : Anti-PGAM1 antibody (ab96622) at 1/1000 dilution
Lane 1 :Recombinant Human PGAM1 protein (ab100885) at 0.1 µg
Lane 2 :Recombinant Human PGAM1 protein (ab100885) at 0.01 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique
Performed under reducing conditions.
Exposure time : 1 minute
プロトコール
Anti-PGAM1 antibody (ab96622) 使用論文
ab96622 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"