製品の概要

  • 製品名Anti-PGAM1 antibody
    PGAM1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to PGAM1
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Zebrafish
  • 免疫原

    Recombinant fragment, corresponding to a region within amino acids 16-209 of Human PGAM1 (AAH00455).

  • ポジティブ・コントロール
    • 293T, A431, Jurkat and Raji cell lysates

法規制情報

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab96622 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/3000. Predicted molecular weight: 29 kDa.

ターゲット情報

  • 機能Interconversion of 3- and 2-phosphoglycerate with 2,3-bisphosphoglycerate as the primer of the reaction. Can also catalyze the reaction of EC 5.4.2.4 (synthase) and EC 3.1.3.13 (phosphatase), but with a reduced activity.
  • 組織特異性In mammalian tissues there are two types of phosphoglycerate mutase isozymes: type-M in muscles and type-B in other tissues.
  • 配列類似性Belongs to the phosphoglycerate mutase family. BPG-dependent PGAM subfamily.
  • Information by UniProt
  • 参照データベース
  • 別名
    • BPG dependent PGAM 1 antibody
    • BPG dependent PGAM1 antibody
    • BPG-dependent PGAM 1 antibody
    • OTTHUMP00000059414 antibody
    • PGAM 1 antibody
    • PGAM A antibody
    • PGAM B antibody
    • PGAM-B antibody
    • PGAM1 antibody
    • PGAM1_HUMAN antibody
    • PGAMA antibody
    • PGAMB antibody
    • Phosphoglycerate mutase 1 antibody
    • Phosphoglycerate mutase 1 brain antibody
    • Phosphoglycerate mutase A antibody
    • Phosphoglycerate mutase A nonmuscle form antibody
    • Phosphoglycerate mutase isozyme B antibody
    see all

Anti-PGAM1 antibody 画像

  • All lanes : Anti-PGAM1 antibody (ab96622) at 1/1000 dilution

    Lane 1 : 293T whole cell lysate
    Lane 2 : A431 whole cell lysate
    Lane 3 : Jurkat whole cell lysate
    Lane 4 : Raji whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 29 kDa
  • All lanes : Anti-PGAM1 antibody (ab96622) at 1/1000 dilution

    Lane 1 : Recombinant Human PGAM1 protein (ab100885) at 0.1 µg
    Lane 2 : Recombinant Human PGAM1 protein (ab100885) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 1 minute

Anti-PGAM1 antibody (ab96622) 使用論文

ab96622 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Jurkat Cells)
Loading amount 30 µg
Specification Jurkat Cells
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

投稿 Aug 10 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"