Anti-PFKFB4 抗体 (ab71622)

製品の概要

  • 製品名Anti-PFKFB4 antibody
    PFKFB4 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to PFKFB4
  • アプリケーション適用あり: WB, ELISA, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide corresponding to Human PFKFB4 (internal sequence) conjugated to Keyhole Limpet Haemocyanin (KLH).

  • ポジティブ・コントロール
    • Formalin-fixed, paraffin-embedded human hepatocarcinoma tissue and mouse brain tissue lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab71622 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
ELISA
IHC-P
  • 追加情報ELISA: 1/1000.
    IHC-P: 1/50 - 1/100.
    WB: 1/100 - 1/500. Detects a band of approximately 60 kDa and many other bands (predicted molecular weight: 54 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    Anti-PFKFB4 antibody 画像

    • Anti-PFKFB4 antibody (ab71622) at 1/100 dilution + mouse brain tissue lysate at 12.5 µg

      Predicted band size : 54 kDa
      Observed band size : 60 kDa (why is the actual band size different from the predicted?)
    • Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with ab71622 at a 1:50 dilution. A peroxidase-conjugated secondary antibody was then used, followed by AEC staining.
    • Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with ab71622 at 1:50 dilution. A peroxidase-conjugated secondary antibody was then used, followed by DAB staining.
    • ICC/IF image of ab71622 stained HepG2 cells. The cells were 4% PFA fixed (10mins) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab71622, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    Anti-PFKFB4 antibody (ab71622) 使用論文

    ab71622 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you and your customer for taking the time to complete our questionnaire and contact us. I am sorry to hear your customer has had difficulty obtaining satisfactory results from these antibodies.

    The details your customer has kindly provi...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (HCT116)
    Loading amount 25 µg
    Specification HCT116
    Gel Running Conditions Reduced Denaturing (10%)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Username

    Abcam user community

    Verified customer

    投稿 Nov 23 2010

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Mouse Cell lysate - whole cell (NIH 3T3 cells)
    Loading amount 25 µg
    Specification NIH 3T3 cells
    Gel Running Conditions Reduced Denaturing (10%)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Username

    Abcam user community

    Verified customer

    投稿 Nov 23 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"