製品の概要
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製品名Anti-PFKFB3 antibody
PFKFB3 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to PFKFB3
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アプリケーション適用あり: IP, IHC-P, WBmore details
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種交差性交差種: Human
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免疫原
Synthetic peptide, corresponding to a region within amino acids 457 and 520 of Human PFKFB3 (NP_004557).
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ポジティブ･コントロール
- A431, H1299, HeLa, HepG2 This antibody gave a positive result in IHC in the following FFPE tissue: Human breast fibroadenoma.
法規制情報
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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バッファーPreservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol, 0.1M Tris, 0.1M Glycine, pH 7 -
Concentration information loading... -
精製度Immunogen affinity purified
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
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Positive Controls
アプリケーション
Our Abpromise guarantee covers the use of ab96699 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IP | Use at an assay dependent concentration. | |
| IHC-P | Use a concentration of 5 µg/ml. | |
| WB | 1/500 - 1/3000. Predicted molecular weight: 60 kDa. |
ターゲット情報
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機能Synthesis and degradation of fructose 2,6-bisphosphate.
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組織特異性Ubiquitous.
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配列類似性In the C-terminal section; belongs to the phosphoglycerate mutase family.
- Information by UniProt
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参照データベース
- Entrez Gene: 5207 Human
- Entrez Gene: 5208 Human
- Entrez Gene: 5209 Human
- Entrez Gene: 5210 Human
- Omim: 605319 Human
- SwissProt: O60825 Human
- SwissProt: P16118 Human
- SwissProt: Q16875 Human
see all -
別名
- 6 phosphofructo 2 kinase/ fructose 2,6 bisphosphatase antibody
- 6 phosphofructo 2 kinase/fructose 2,6 biphosphatase 3 antibody
- 6-bisphosphatase antibody
see all
Anti-PFKFB3 antibody 画像
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Western blot - PFKFB3 antibody (ab96699)All lanes : Anti-PFKFB3 antibody (ab96699) at 1/1000 dilution
Lane 1 : A431 whole cell lysate
Lane 2 : H1299 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size : 60 kDa -
Immunoprecipitation - Anti-PFKFB3 antibody (ab96699)PFKFB3 was immunoprecipitated using 0.5mg A431 whole cell extract, 5µg of Rabbit polyclonal to PFKFB3 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, A431 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab96699.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 70kDa: PFKFB3. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PFKFB3 antibody (ab96699)IHC image of PFKFB3 staining in Human breast fibroadenoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab96699, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Anti-PFKFB3 antibody (ab96699) 使用論文
This product has been referenced in:
- Li Z et al. Plk1 phosphorylation of PTEN causes a tumor-promoting metabolic state. Mol Cell Biol 34:3642-61 (2014). Read more (PubMed: 25047839) »
- Esen E et al. WNT-LRP5 signaling induces Warburg effect through mTORC2 activation during osteoblast differentiation. Cell Metab 17:745-55 (2013). Read more (PubMed: 23623748) »
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"