製品の概要

  • 製品名Anti-PFKFB1 antibody
    PFKFB1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to PFKFB1
  • アプリケーション適用あり: ELISA, IHC-P, WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide from the center region of human PFKFB1 conjugated to KLH

  • ポジティブ・コントロール
    • Jurkat cell line lysate and human hepatocarcinoma tissue.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab71625 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/1000.
IHC-P 1/50 - 1/100.
WB 1/100 - 1/500. Detects a band of approximately 65 kDa (predicted molecular weight: 55 kDa).

ターゲット情報

Anti-PFKFB1 antibody 画像

  • Anti-PFKFB1 antibody (ab71625) at 1/240 dilution + Jurkat cell line lysate at 35 µg

    Predicted band size : 55 kDa
    Observed band size : 65 kDa (why is the actual band size different from the predicted?)
  • ab71625, at 1/50 dilution, staining PFKFB1 in formalin fixed, paraffin embedded human hepatocarcinoma tissue by Immunohistochemistry. ab71625 was peroxidase conjugated to the secondary antibody, followed by DAB staining.

Anti-PFKFB1 antibody (ab71625) 使用論文

This product has been referenced in:
  • Storm P  et al. Conserved features of cancer cells define their sensitivity to HAMLET-induced death; c-Myc and glycolysis. Oncogene 30:4765-79 (2011). WB . Read more (PubMed: 21643007) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HEK293)
Loading amount 15 µg
Specification HEK293
Gel Running Conditions Reduced Denaturing ('any Kd' Biorad precast)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Christoph Schmitt

Verified customer

投稿 Jul 28 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"