The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/50 - 1/100. Detects a band of approximately 15 kDa (predicted molecular weight: 15 kDa). Also detects a range of bands 28-72kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it. Binds to nascent polypeptide chain and promotes folding in an environment in which there are many competing pathways for nonnative proteins.