製品の概要

  • 製品名Anti-PFD1145c antibody
    PFD1145c 一次抗体 製品一覧
  • 製品の詳細
    Mouse polyclonal to PFD1145c
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Plasmodium falciparum
  • 免疫原

    Recombinant fusion protein containing a His tag:

    HPYDINNKNDDSYRYDISEE

    , corresponding to amino acids 240-259 of Plasmodium falciparum PFD1145c.

  • 特記事項


    This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: 50% Glycerol, Whole serum
  • 精製度Whole antiserum
  • 一次抗体 備考This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab52699 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000.

This antibody has been tested in Western blot against an E.coli lysate containing the partial recombinant fusion protein used as an immunogen. We have no data on detection of endogenous protein.

ターゲット情報

  • 関連性PFD1145c is a putative uncharacterized protein from Plasmodium falciparum>.
  • 参照データベース
    • 別名
      • Hypothetical protein PFD1145c antibody

    Anti-PFD1145c antibody 画像

    • All lanes : Anti-PFD1145c antibody (ab52699) at 1/1000 dilution

      Lane 1 : ~20µg of a total protein extract from E coli with ~50ng to 100ng of a recombinant fusion protein of an irrelevant antigen
      Lane 2 : ~20µg of a total protein extract from E coli with ~50ng to 500ng of the antigen (recombinant fusion protein containing a His tag)

      Secondary
      Rabbit anti-mouse IgG + IgM, (H+L) horseradish peroxidase conjugated at 1/5000 dilution

    Anti-PFD1145c antibody (ab52699) 使用論文

    ab52699 has not yet been referenced specifically in any publications.

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