製品の概要

  • 製品名Anti-PFAS antibody
    PFAS 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to PFAS
  • アプリケーション適用あり: ELISA, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Horse, Guinea pig, Cat
  • 免疫原

    Synthetic peptide corresponding to a region within N terminal amino acids 252-301 (ESIMSTQESS NPNNVLKFCD NSSAIQGKEV RFLRPEDPTR PSRFQQQQGL) of Human PFAS (NP_036525).

  • ポジティブ・コントロール
    • 293T cell lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab82755 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent concentration.

Titre using peptide based assay: 1/312500.

WB Use a concentration of 1 µg/ml. Predicted molecular weight: 145 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 関連性Purines are necessary for many cellular processes, including DNA replication, transcription, and energy metabolism. Ten enzymatic steps are required to synthesize inosine monophosphate (IMP) in the de novo pathway of purine biosynthesis. PFAS catalyzes the fourth step of IMP biosynthesis.
  • 細胞内局在Cytoplasmic
  • 参照データベース
  • 別名
    • PFAS antibody
    • FGAM synthase antibody
    • FGAMS antibody
    • FGAR amidotransferase antibody
    • FGARAT antibody
    • Formylglycinamide ribotide amidotransferase antibody
    • Formylglycinamide ribotide synthetase antibody
    • KIAA0361 antibody
    • Phosphoribosylformylglycinamidine synthase antibody
    • PURL antibody
    see all

Anti-PFAS antibody 画像

  • Anti-PFAS antibody (ab82755) at 1 µg/ml (in 5% skim milk / PBS buffer) + 293T cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 145 kDa
    Observed band size : 145 kDa
  • All lanes : Anti-PFAS antibody (ab82755) at 1/2000 dilution

    Lane 1 : Human Skin Fibroblasts at 100 µg
    Lane 2 : HEK273 cells at 20 µg
    Lane 3 : HeLa cells at 20 µg


    Predicted band size : 145 kDa

Anti-PFAS antibody (ab82755) 使用論文

This product has been referenced in:
  • French JB  et al. Hsp70/Hsp90 chaperone machinery is involved in the assembly of the purinosome. Proc Natl Acad Sci U S A 110:2528-33 (2013). Read more (PubMed: 23359685) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"