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Our Abpromise guarantee covers the use of ab9561 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration. Detects a band of approximately 11 kDa (predicted molecular weight: 11 kDa). To detect hPF-4 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hPF-4 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.|
|ELISA||Use at an assay dependent concentration. To detect hPF-4 by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hPF-4.|
|Neutralising||Use at an assay dependent concentration. To yield one-half maximal inhibition [ND50] of the biological activity of hPF-4 (50 ng/ml), a concentration of 1.75 - 3.0 µg/ml of this antibody is required.|
|Sandwich ELISA||Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit polyclonal to PF4 (Biotin) (ab83123).
Can be paired for Sandwich ELISA with ab83123