製品の概要

  • 製品名Anti-PEX11B antibody
    PEX11B 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to PEX11B
  • 特異性ab74507 detects endogenous levels of total PEX11B protein.
  • アプリケーション適用あり: ICC/IF, IHC-P, WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    Synthesized peptide derived from an internal region of human PEX11B.

  • ポジティブ・コントロール
    • Extracts from COLO cells, Jurkat cells, HUVEC cells and COS-7 cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab74507 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB 1/500 - 1/1000. Predicted molecular weight: 28 kDa.
ELISA 1/10000.

ターゲット情報

Anti-PEX11B antibody 画像

  • All lanes : Anti-PEX11B antibody (ab74507) at 1/500 dilution

    Lane 1 : extracts from COLO cells
    Lane 2 : extracts from Jurkat cells
    Lane 3 : extracts from HUVEC cells
    Lane 4 : extracts from COS-7 cells
    Lane 5 : extracts from COLO cells with immunising peptide at 10 µg

    Lysates/proteins at 10 µg per lane.


    Predicted band size : 28 kDa
    Observed band size : 28 kDa
    Additional bands at : 48 kDa,55 kDa,75 kDa. We are unsure as to the identity of these extra bands.
  • ICC/IF image of ab74507 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab74507, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • IHC image of ab74507 staining in human normal colon formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH9, epitope retrieval solution 2) for 20 mins. The section was then incubated with ab74507, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Anti-PEX11B antibody (ab74507) 使用論文

This product has been referenced in:
  • Amaral A  et al. Human sperm tail proteome suggests new endogenous metabolic pathways. Mol Cell Proteomics 12:330-42 (2013). ICC/IF ; Human . Read more (PubMed: 23161514) »
  • Bonekamp NA  et al. Self-interaction of human Pex11pß during peroxisomal growth and division. PLoS One 8:e53424 (2013). WB, ICC/IF ; Human . Read more (PubMed: 23308220) »

See all 4 Publications for this product

Product Wall

Application Western blot
Sample Rat Cell lysate - other (cultured AR42J cells, pancreatic acinar, rat)
Loading amount 60 µg
Specification cultured AR42J cells, pancreatic acinar, rat
Gel Running Conditions Reduced Denaturing (12.5% PAA)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Dr. Michael Schrader

Verified customer

投稿 Apr 19 2011

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample African Green Monkey Cell (COS-7 cells, kidney epithelial cells)
Specification COS-7 cells, kidney epithelial cells
Fixative Formaldehyde
Permeabilization Yes - Triton-X 100 or Digitonin
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 20°C
Username

Dr. Michael Schrader

Verified customer

投稿 Apr 19 2011

Application Immunocytochemistry/ Immunofluorescence
Sample African Green Monkey Cultured Cells (COS-7 kidney epithelial cells)
Specification COS-7 kidney epithelial cells
Fixative Methanol
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 20°C
Username

Dr. Michael Schrader

Verified customer

投稿 Apr 19 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - other (HepG2 cells, liver peroxisome-enriched fraction)
Loading amount 60 µg
Specification HepG2 cells, liver peroxisome-enriched fraction
Gel Running Conditions Reduced Denaturing (12.5% PAA)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Dr. Michael Schrader

Verified customer

投稿 Apr 14 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"