製品の概要

  • 製品名
  • 製品の詳細
    Goat polyclonal to Pescadillo
  • 由来種
    Goat
  • アプリケーション
    適用あり: ELISA, ICC, WB, EMSAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Cow, Pig
  • 免疫原

    Synthetic peptide:

    GGLEKKKYERGSA

    , corresponding to N terminal amino acids 2-14 of Human Pescadillo.

  • ポジティブ・コントロール
    • Hela lysate.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline. pH 7.3
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • 特記事項(精製)
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab19036 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/32000. Peptide ELISA
ICC Use a concentration of 2.5 - 3.8 µg/ml.
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 68 kDa).
EMSA Use at an assay dependent dilution.

ターゲット情報

  • 機能
    Component of the PeBoW complex, which is required for maturation of 28S and 5.8S ribosomal RNAs and formation of the 60S ribosome.
  • 組織特異性
    Significant levels are detected in a variety of cancer cell lines, including glioblastoma, breast carcinoma, colon carcinoma and cervical carcinoma cells. Levels are abnormally elevated in malignant tumors of astrocytic origin.
  • 配列類似性
    Belongs to the pescadillo family.
    Contains 1 BRCT domain.
  • 翻訳後修飾
    Sumoylated.
  • 細胞内局在
    Nucleus > nucleolus. Nucleus > nucleoplasm. Chromosome. Appears to localize to the periphery of metaphase chromosomes during mitosis and to the prenucleolar bodies that form in mitotic cells prior to the actual nucleoli.
  • Information by UniProt
  • 参照データベース
  • 別名
    • FLJ43790 antibody
    • Homo sapiens pescadillo mRNA complete cds antibody
    • PES antibody
    • PES 1 antibody
    • pes1 antibody
    • PESC_HUMAN antibody
    • Pescadillo (zebrafish) homolog 1 containing BRCT domain antibody
    • Pescadillo homolog 1 antibody
    • Pescadillo homolog 1 containing BRCT domain (zebrafish) antibody
    • Pescadillo homolog antibody
    • Pescadillo ribosomal biogenesis factor 1 antibody
    see all

画像

  • ab19036 (1µg/ml) staining of Hela lysate (35µg protein in RIPA buffer) approx. 70kDa.  Primary incubation was 1 hour.  Detected by chemiluminescence.

    ab19036 (1µg/ml) staining of Hela lysate (35µg protein in RIPA buffer) approx. 70kDa. Primary incubation was 1 hour. Detected by chemiluminescence.
  • ab19036 (3.8µg/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

参考文献

This product has been referenced in:
  • Sikorski EM  et al. Pescadillo interacts with the cadmium response element of the human heme oxygenase-1 promoter in renal epithelial cells. J Biol Chem 281:24423-30 (2006). Read more (PubMed: 16816389) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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