製品の概要

  • 製品名Anti-PERP antibody
    PERP 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to PERP
  • アプリケーション適用あり: ELISA, WB, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Cow, Dog, Xenopus laevis, Chimpanzee
  • 免疫原

    Synthetic peptide:

    CLPNYEDDLLGNAK

    from the C Terminus of the protein sequence (Human)

  • ポジティブ・コントロール
    • Human Liver lysate

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab48032 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent dilution.
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 21 kDa (predicted molecular weight: 20 kDa).
IHC-P 1/100. (see Abreview)

ターゲット情報

  • 機能Component of intercellular desmosome junctions. Plays a role in stratified epithelial integrity and cell-cell adhesion by promoting desmosome assembly. Plays a role as an effector in the TP53-dependent apoptotic pathway.
  • 組織特異性Expressed in skin, heart, placental, liver, pancreas, keratinocytes and dermal fibroblasts.
  • 配列類似性Belongs to the TMEM47 family.
  • 細胞内局在Cell junction > desmosome. Cell membrane. Associated with desmosomes.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 1110017A08Rik antibody
    • dJ496H19.1 antibody
    • KCP 1 antibody
    • KCP-1 antibody
    • KCP1 antibody
    • Keratinocyte associated protein 1 antibody
    • Keratinocyte-associated protein 1 antibody
    • Keratinocytes associated protein 1 antibody
    • KRTCAP 1 antibody
    • KRTCAP1 antibody
    • p53 apoptosis effector related to PMP 22 antibody
    • p53 apoptosis effector related to PMP-22 antibody
    • p53 apoptosis effector related to PMP22 antibody
    • P53 induced protein PIGPC1 antibody
    • P53-induced protein PIGPC1 antibody
    • Perp antibody
    • PERP TP53 apoptosis effector antibody
    • PERP_HUMAN antibody
    • PIGPC 1 antibody
    • PIGPC1 antibody
    • RP3 496H19.1 antibody
    • THW antibody
    • TP53 apoptosis effector antibody
    • Transmembrane protein THW antibody
    see all

Anti-PERP antibody 画像

  • Anti-PERP antibody (ab48032) at 0.3 µg/ml + Human Liver lysate in RIPA buffer at 35 µg

    Predicted band size : 20 kDa
    Observed band size : 21 kDa (why is the actual band size different from the predicted?)
  • ab48032 staining PERP in human small intestine tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded tissue sections). The sections were fixed in paraformaldehyde prior to blocking with 10% serum for 1 hour at 20°C. The primary antibody was diluted 1/100 and incubated with the sample for 12 hours at 4°C. An HRP-conjugated rabbit anti-goat polyclonal was used as the secondary antibody, diluted 1/200.

    See Abreview

Anti-PERP antibody (ab48032) 使用論文

This product has been referenced in:
  • Gomez-Sanchez JC  et al. The human Tp53 Arg72Pro polymorphism explains different functional prognosis in stroke. J Exp Med 208:429-37 (2011). WB ; Human . Read more (PubMed: 21357744) »

See 1 Publication for this product

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Thank you for your email and for keeping me updated about the situation.
I'm sorry to hear that ab5986 did not work well with the mouse samples, and it is possible that this antibody just doesn't work with mouse. We have unfortunately removed ab39...

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Thank you for your call today and for your interest in ab48032. Please see below for the details of your testing discount code, and let me know if you have any questions or if there is anything else that we can do for you.
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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Small intestine)
Specification Small intestine
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
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投稿 May 28 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"