製品の概要

  • 製品名Anti-Peroxiredoxin-SO3 antibody
    Peroxiredoxin-SO3 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Peroxiredoxin-SO3
  • アプリケーション適用あり: WB, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic sulfonylated peptide (Human) conjugated to KLH, corresponding to Prx I-IV active site.

  • ポジティブ・コントロール
    • H2O2 treated HeLa whole cell lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab16830 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000. Predicted molecular weight: 22 kDa.
IHC-P 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use at an assay dependent concentration. PubMed: 18826942

ターゲット情報

  • 関連性Peroxiredoxin (Prx) is an antioxidant enzyme detoxifying reactive oxygen species and has a cysteine at the active site. Prx enzymes modulate various receptor signaling pathways and protect cells from oxidatively induced death. Peroxiredoxin 1 to 4 have two conserved Cys residues corresponding to Cys51 and Cys172 of mammalian Peroxiredoxin 1. The active site cysteine(Cys51) is oxidized to cysteine sulfenic acid(Cys51-SOH) when a peroxide is reduced. Because Cys51-SOH is unstable, it forms a disulfide with Cys172-SH which comes from the other subunit of the homodimer. The disulfide is then reduced back to the Prx active thiol form by the thioredoxin-thioredoxin reductase system. However, the formation of the disulfide is a slow process. Thus under oxidative stress conditions, the sulfenic intermediate(Cys51-SOH) can be easily over oxidized to cysteine sulfinic acid(Cys-SO2H) or cysteine sulfonic acid(Cys-SO3H) before it is able to form a disulfide. Recent studies suggest that over oxidized Prx can be reduced back to the active form during recovery after oxidative stress.
  • 細胞内局在Cytoplasmic
  • 参照データベース
  • 別名
    • Peroxiredoxin 1 antibody
    • Peroxiredoxin 2 antibody
    • Peroxiredoxin 3 antibody
    • Peroxiredoxin 4 antibody
    • PRDX1 antibody
    • PRDX2 antibody
    • PRDX3 antibody
    • PRDX4 antibody
    see all

Anti-Peroxiredoxin-SO3 antibody 画像

  • IHC image of ab16830 staining in human hippocampus formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab16830,1/1000 dilution, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.



  • Predicted band size : 22 kDa

    Upper blot - Prx-SO3 antibody (ab16830) at 1/2000
    Lower blot - Prx1 antibody as a loading control

    Lane 1: H2O2 untreated HeLa cell lysate
    Lane 2: H2O2 treated HeLa cell lysate
    Lane 3: Reduced recombinant Prx1
    Lane 4: Oxidized recombinant Prx1

  •  

Anti-Peroxiredoxin-SO3 antibody (ab16830) 使用論文

This product has been referenced in:
  • Causton HC  et al. Metabolic Cycles in Yeast Share Features Conserved among Circadian Rhythms. Curr Biol 25:1056-62 (2015). WB ; Saccharomyces cerevisiae . Read more (PubMed: 25866393) »
  • Homma T  et al. SOD1 deficiency induces the systemic hyperoxidation of peroxiredoxin in the mouse. Biochem Biophys Res Commun 463:1040-6 (2015). Read more (PubMed: 26079888) »

See all 20 Publications for this product

Product Wall

Application Western blot
Sample Rat Tissue lysate - whole (Aorta)
Gel Running Conditions Non-reduced Denaturing
Loading amount 50 µg
Specification Aorta
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Dr. D Richard

Verified customer

投稿 Jul 27 2015

Application Western blot
Sample Saccharomyces cerevisiae Cell lysate - whole cell (TCA prep)
Gel Running Conditions Reduced Denaturing (4 -12% NuPAGE bis-tris gel with MES buffer and antioxidant)
Loading amount 10 µg
Treatment With or without 1mM H2O2 for 15 minutes
Specification TCA prep
Blocking step 0.5% BSA and 0.5% milk in TBST as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.5% · Temperature: 20°C
Username

Dr. Helen Causton

Verified customer

投稿 Apr 22 2015

Application IHC - Wholemount
Sample Rat Tissue (Mesenteric Venule)
Specification Mesenteric Venule
Username

Abcam user community

Verified customer

投稿 Dec 18 2012

Thank you for your enquiry.

We do not have set expiration dates for our products. Most antibodies are stable and can last for anywhere from a few months to several years if stored properly, so we strongly recommend that you follow the storage ...

Read More

Thank you for contacting Abcam and I am sorry for the late response.
I have talked to the lab about the protocol that was used to oxidize the PrX1 protein (ab16830) and they recommended fallowing the protocol in the attached reference.
Please...

Read More

Thank you for contacting us with your questions. We have only tested these antibodies under reducing conditions in WB. They may be suitable for use under non-reducing conditions as well, but this has not been validated in-house. I hope this information...

Read More

Thank you for contacting us.  We have only tested these Peroxiredoxin antibodies under reducing conditions in WB.  They may be suitable for use under non-reducing conditions as well, but this has not been validated in house.  The unconjugated secondary...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - whole cell (Mouse Embryonic Fibroblasts)
Loading amount 30 µg
Specification Mouse Embryonic Fibroblasts
Treatment 100 uM H2O2 for 6 hrs
Gel Running Conditions Non-reduced Denaturing (10% SDS)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Feb 16 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Cell lysate - whole cell (primary neurons)
Loading amount 600000 cells
Specification primary neurons
Treatment H2O2 100uM 1h
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

投稿 Dec 21 2006

Thank you for your enquiry. I can confirm that milk was used as a blocking agent to test ab16830 by western blotting. Please let me know if you have any further inquiries.

1-10 of 11 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"